终端脱氧核样转移酶和CD84识别了人类多能淋巴细胞原始体
YeEun Kim1,2, Ariel A Calderon1,2, Patricia Favaro2
1Immunology Graduate Program, Stanford University, Stanford, CA, USA.
Nature communications
|July 13, 2024
概括
研究人员发现了一种新型的人类骨髓前代细胞. 这些淋巴细胞原始原体表达终端脱氧核样转移酶 (TdT),并具有淋巴细胞和骨髓细胞分化潜力.
科学领域:
- 血液形成 血液形成 血液形成
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 来自人类造血细胞原始体的淋巴细胞特征仍然不完全理解.
- 将淋巴细胞身份与原始细胞中的特定蛋白质标记物联系起来,对于推进血液形成研究至关重要.
研究的目的:
- 为了识别与人类骨髓前代的淋巴细胞身份相关的蛋白质水平细胞特征.
- 为了表征新的祖先种群,这些种群弥合了淋巴骨髓发育轴.
主要方法:
- 在人类骨髓原始体上利用了高度多重化的单细胞蛋白质查.
- 进行了染色体可访问性测试,以确定监管动机.
- 对已识别的原始基因子集进行了前性隔离和ex vivo分化试验.
主要成果:
- 在B/T细胞出现之前的CD34+原始体中确定了终端脱氧核样转移酶 (TdT) 表达.
- 发现TdT+细胞具有颗粒细胞-单细胞原体 (GMP) 免疫表型,但富含淋巴细胞相关转录因子 (TF) 动机.
- 证明CD84低的GMPs表现出强大的淋巴细胞潜力ex vivo,同时保持髓状细胞容量.
结论:
- 确定了一个独特的人类骨髓淋巴原始原始体的种群.
- 在GMP中,特征TdT作为早期淋巴细胞特征的新标记.
- 定义了对人类血液形成中的淋巴骨髓轴的更细致的理解.
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