Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

iPS Cell Differentiation01:22

iPS Cell Differentiation

2.7K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Towards the construction of a virtual yeast.

Nature·2026
Same author

Sex Differences in ER Stress Pathways Are a Key Determinant of β-Cell Proliferation and Resilience.

Diabetes·2026
Same author

Author Correction: Long-term, in toto live imaging of cardiomyocyte behaviour during mouse ventricle chamber formation at single-cell resolution.

Nature cell biology·2026
Same author

A reversible broad-spectrum antiviral targets the human V-ATPase V<sub>O</sub> domain.

Research square·2026
Same author

Regenerative macrophages enhance stem cell-derived β cell function and engraftment.

Science advances·2026
Same author

Dual Jamming of Biobased CNC-Stabilized Bijel Fibers via Continuous Solvent Transfer Induced Phase Separation.

ACS applied materials & interfaces·2026
Same journal

Unlocking the capacity of Mn-based Prussian blue cathodes in capacitive deionization.

Nature communications·2026
Same journal

Scaling biodiversity-stability relationships from populations to meta-communities across trophic levels.

Nature communications·2026
Same journal

Thermodynamically programmed one-pot CRISPR platform for point-of-care SNP genotyping.

Nature communications·2026
Same journal

Engineering all-organic electrocatalysts with asymmetric dual-active sites for uncommon oxygen-evolving pathway.

Nature communications·2026
Same journal

Rapid GC content evolution in rice through GC-biased gene conversion and selection for translation efficiency.

Nature communications·2026
Same journal

Declines in organic matter persistence with increased soil carbon.

Nature communications·2026
查看所有相关文章

相关实验视频

Updated: Jun 21, 2025

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
09:33

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Published on: June 2, 2018

9.1K

在干细胞衍生的小岛球形系统中,PDX1+细胞的芽生长形态发生.

Jia Zhao1, Shenghui Liang2, Haoning Howard Cen2

  • 1Life Sciences Institute, Departments of Cellular & Physiological Sciences and Surgery, University of British Columbia, Vancouver, BC, Canada. jia.zhao@ubc.ca.

Nature communications
|July 13, 2024
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种新方法,从人类干细胞中制造胰岛素生产小岛细胞,模仿自然发育并帮助疾病研究.

更多相关视频

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures
08:32

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures

Published on: March 27, 2019

6.1K
Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System
10:12

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

Published on: December 16, 2021

2.7K

相关实验视频

Last Updated: Jun 21, 2025

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
09:33

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Published on: June 2, 2018

9.1K
Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures
08:32

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures

Published on: March 27, 2019

6.1K
Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System
10:12

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

Published on: December 16, 2021

2.7K

科学领域:

  • 干细胞生物学 干细胞生物学
  • 发育生物学是发展生物学.
  • 内分泌学 在内分泌学.

背景情况:

  • 人类多能干细胞 (hPSC) 是产生胰岛素分泌小岛细胞的有希望的来源.
  • 目前的分化协议在复制本地小岛的复杂结构和细胞组成方面存在局限性.

研究的目的:

  • 开发一种可调节的策略,从hPSCs生成模拟自然小岛形态发生的小岛球体.
  • 研究PDX1和EphB3/4信号传递在小岛细胞芽和组织中的作用.
  • 模拟人类胰腺发育和疾病,如米切尔-莱利综合征,使用hPSC衍生小岛.

主要方法:

  • 将PDX1+细胞芽形态发生过程纳入hPSCs的分阶段分化.
  • 在人胰腺发育的体外建模.
  • 产生RFX6淘汰hPSCs以建模米切尔-莱利综合征.

主要成果:

  • 一个可调节的分化系统成功地生成了为主要岛屿细胞类型丰富的小岛球体.
  • 这个过程模仿了自然的小岛形态发生,从而产生了核心或混合的内分泌细胞排列.
  • 确定PDX1和EphB3/4信号传递对细胞芽形态发生至关重要.
  • 在岛屿分化过程中,RFX6淘汰hPSCs表现出显著的形态发生缺陷.

结论:

  • 开发的战略为从hPSCs中生产功能小岛球体提供了一种新的方法.
  • 这种系统有助于我们更好地理解人类小岛的发育以及胰腺相关疾病背后的机制.
  • 来自hPSC的小岛模型是基础小岛生物学研究和疾病建模的宝贵工具.