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基因组组合的染色体水平的Hippophae rhamnoides品种

Xingyu Yang^1,2, Shujie Luo^1,3, Shihai Yang⁴

¹Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, kunming, 650000, China.

Scientific data

|July 13, 2024

概括

我们对Fructus hippophae的染色体级基因组进行了测序,这是一种有价值的海杂交. 这为了解其经济和医疗潜力以及未来海棘研究提供了关键的基因组数据.

科学领域:

植物基因组学植物基因组学
生物信息学是一种生物信息学.
农业科学农业科学

背景情况:

海的杂交品种Fructus hippophae具有重要的经济和药用价值.
了解它的遗传构成对于进一步的开发和研究至关重要.

研究的目的:

为了生成Fructus hippophae的染色体级基因组组合.
为海松泛基因组学和性别差异化研究提供基因组资源.

主要方法:

染色体层次的基因组测序和组装 (918.59 Mb).
基因结构预测使用de-novo,同质性和转录组组装.
使用BUSCO和映射率进行质量评估.

主要成果:

组装N50达到83.65Mb,其中97.17%的序列固定在12个伪染色体上.
预测了36475个编码蛋白质的基因,其中36226个功能性注释.
63.68%的基因组由重复元素组成;148,3600 bp的非编码RNA注释.

结论:

获得了女性Fructus hippophae的高质量基因组组.
这种基因组信息将有助于泛基因组构造和了解海棘中的性别差异化.

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相关概念视频

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.