相关实验视频

Updated: Jun 21, 2025

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

提高CRISPR/Cas9中介基因组编辑在果收获后病原体Penicillium digitatum中的效率

Carolina Ropero-Pérez¹, Jose F Marcos¹, Paloma Manzanares¹

¹Food Biotechnology Department, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Catedrático Agustín Escardino Benlloch 7, Paterna, Valencia, 46980, Spain.

Fungal biology and biotechnology

|July 13, 2024

概括

我们通过修改选择方法,将青菌病原体Penicillium digitatum的CRISPR/Cas9基因编辑效率从10%提高到80%以上. 这一突破为这种经济重要真菌提供了实际的基因工程.

科学领域:

农业科学农业科学
菌类学菌类学是指菌类学.
分子生物学分子生物学

背景情况:

虫 (Penicillium digitatum) 在类水果中引起绿色菌病,影响农业经济.
之前在P. digitatum中进行的CRISPR/Cas9基因编辑仅实现了10%的效率,限制了实际应用.

研究的目的:

为了显著提高CRISPR/Cas9介导的基因编辑效率在P. digitatum.
为了提高这种植物病原菌的基因工程可行性.

主要方法:

通过在特定生长条件下增加培养时间来修改选择方法.
在P. digitatum中破坏了五个候选基因,使用了优化的CRISPR/Cas9协议.

主要成果:

实现了基因编辑效率的大幅提高,从54%到83%不等.
成功破坏基因,包括具有视觉表型变化的基因 (pksP,arp2) 和没有明显表型的基因 (PDIG_56860,rodA,dfg5).

结论:

修改的选择方法极大地提高了P. digitatum基因组编辑效率.
这种优化的CRISPR/Cas9方法对于基因破坏是可行的,并且适用于其他编辑效率低的真菌.

关键词:

插曲式表达向量是一个插曲式表达向量.状真菌是一种状真菌.基因准是基因的目标.非模型真菌的非模型真菌.

更多相关视频

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...