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胚胎热操纵通过调节短暂受体潜在V2表达的调节热应激肉的骨肌中的线粒体功能
Sheng Li1, Xiaoqing Li1, Kai Wang1
1Research Centre for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Poultry science
|July 14, 2024
概括
产前热操纵通过上调TRPV2表达和增强电子运输链活动,改善肉肌肉发育和热应激下的线粒体功能.
科学领域:
- 动物科学动物科学
- 生理学 生理学 生理学
- 生物化学 生物化学
背景情况:
- 热应激对家禽的骨肌肉发育和线粒体功能产生负面影响.
- 线粒体功能障碍是降低在热应激下家禽生产效率的关键因素.
- 暂时受体潜在瓦尼洛伊德2 (TRPV2) 涉及到细胞应激反应.
研究的目的:
- 研究胚胎热操纵 (TM) 在热应激下对 brojler pectoralis major (PM) 肌肉线粒体功能的影响.
- 探索TRPV2在调解TM对肌肉发育和线粒体功能影响中的作用.
- 评估TRPV2调制对初级髓管中线粒体功能的体外影响.
主要方法:
- 肉从化后的22日至28天受到热中性或循环热应激.
- 胚胎接受了热处理 (41.5°C,65%RH,16-18岁胚胎年龄期间每天3小时).
- 分析了PM肌肉基因表达 (线粒体电子运输链,TRPV2),纤维形态和体外线粒体功能.
主要成果:
- TM没有影响体重或食摄入量,但改善了直肠温度调节和热应激下的T3水平.
- TM促进了PM肌肉纤维的发展,增加了直径和横截面积.
- TM上调了线粒体电子运输链 (ETC) 基因和PM肌肉中的TRPV2表达,显示出正相关性.
- 在体外,TRPV2激活增强了线粒体ETC基因表达和通过增加细胞内的氧化酸化,而抑制逆转了这些效应.
结论:
- 产前热操纵增强肉骨肌肉发育和在热应激下线粒体功能.
- 该机制涉及TRPV2表达的上调和改善线粒体电子运输链活动.
- TRPV2在调节骨肌肉线粒体功能和发育中起着至关重要的作用,以应对热应激.
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