相关实验视频

Updated: Jun 21, 2025

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells

Published on: March 3, 2015

13.3K

介绍功能障碍的蛋白质-蛋白质相互作用体 (dfPPI) - 在疾病中进行系统级蛋白质-蛋白质相互作用 (PPI) 功能障碍调查的平台

Souparna Chakrabarty1, Shujuan Wang1, Tanaya Roychowdhury1

  • 1Chemical Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, 10065, USA.

Current opinion in structural biology
|July 14, 2024
PubMed
概括

功能障碍的蛋白质-蛋白质相互作用体 (dfPPI) 平台在细胞应激下检测蛋白质相互作用的变化. 这种方法有助于了解疾病机制,并开发用于癌症和神经退行性疾病的新疗法.

更多相关视频

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

3.1K
Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

8.0K

相关概念视频

Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
3.9K
The Proteasome01:13

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
827
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.3K
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

3.7K