使用全基因组测序来表征Listeria spp. 农场奶制品加工设施及其相关农场环境中的持久性和传播模式
Samantha Bolten1, Robert D Ralyea2, Timothy T Lott2
1Milk Quality Improvement Program, Department of Food Science, Cornell University, Ithaca, NY 14853; Food Safety Laboratory, Department of Food Science, Cornell University, Ithaca, NY 14853.
Journal of dairy science
|July 14, 2024
概括
农场乳制品加工设施可以减少Listeria spp. 通过实施有针对性的清洁,区划和设计干预措施来减少污染. 这些策略显著降低了加工环境中的李斯特菌检测,减轻了农场环境风险.
科学领域:
- 食品安全 食品安全
- 微生物学 微生物学
- 乳制品科学 乳制品科学
背景情况:
- 农场乳制品加工环境 (PE) 容易受到Listeria spp.的感染. 由于靠近农场环境 (FE) 的污染.
- 有效控制Listeria spp. 的有效控制. 在农场的乳制品需要了解污染源和实施有针对性的干预措施.
研究的目的:
- 评估干预措施对Listeria spp.的影响. 在农场奶制品加工环境中进行检测.
- 评估农场环境作为Listeria spp.的潜在来源. 在加工环境中的污染.
- 为了调查Listeria spp.的潜在传播途径. 在农场和加工环境之间.
主要方法:
- 干预措施的实施:改善清洁/卫生,卫生区划和卫生设备/设施设计.
- 在一年内在加工和农场环境中进行环境采样.
- 李斯特菌种群的全基因组测序 (WGS) 隔离以确定遗传关系和潜在的传播.
主要成果:
- 李斯特里亚菌种类. 在干预一年后,处理环境中的检测率从50%降至7.5%.
- 李斯特里亚菌种类. 在农场环境中检测率更高 (75%),而不是加工环境 (24%).
- 在WGS中发现了共享的Listeria spp. 农场和加工环境之间的集群,表明可能的传播,集群2的分离物与奶牛场水库有关.
结论:
- 有针对性的干预措施有效地减少了Listeria spp. 农场奶制品加工环境中的污染.
- 农场环境是Listeria spp.的重要潜在来源. 对于加工区而言.
- 了解和控制从农场到加工环境的传播对于乳制品食品安全至关重要.
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