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长期暴露于聚乙烯微塑料诱导了通过NF-κB通路减少LHR和降低丸激素水平

Haibo Jin¹, Jie Ding², Hongru Liu²

¹State Key Laboratory of Analytical Chemistry for Life Science, Division of Anatomy and Histo-embryology, Medical School, Nanjing University, Nanjing, Jiangsu, 210093, China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, Jiangsu, 210093, China; School of Public Health, Yangzhou University, Yangzhou, Jiangsu, 225000, China.

Environmental pollution (Barking, Essex : 1987)

|July 14, 2024

概括

聚烯微塑料 (PS-MPs) 暴露会通过降低丸激素而损害男性生殖功能. PS-MPs激活巨细胞,释放TNF-α,这抑制了莱迪格细胞中的LHR转录,降低了的产生.

科学领域:

环境毒理学环境毒理学
生殖生物学生殖生物学
细胞信号传输细胞信号传输

背景情况:

微塑料 (MP) 污染是一个日益严重的环境问题,越来越多的证据表明它们通过食物链转移到哺乳动物身上.
之前的研究将聚乙烯微塑料 (PS-MP) 暴露与男性生殖功能障碍联系起来,特别是降低丸激素水平.
通过PS-MPs影响的精确分子机制,特别是莱迪格细胞中化激素受体 (LHR) 表达,仍然不清楚.

研究的目的:

阐明PS-MPs降低LHR表达并随后影响小鼠莱迪格细胞中的合成和分泌的分子机制.
调查巨细胞激活和极化在丸间隙中介PS-MP毒性的作用.

主要方法:

小鼠在饮用水中暴露在0.5,4或10微米PS-MPs (1000微克/升) 中,持续180天.
在丸组织内分析巨细胞的增殖和M1极化.
研究巨细胞释放的TNF-α及其对莱迪格细胞NF-κB信号通路的影响.
评估LHR促进体结合和随后的LHR基因转录.

主要成果:

暴露于PS-MP导致巨细胞的增殖和偏向M1表型.
巨细胞释放瘤坏死因子α (TNF-α),激活莱迪格细胞中的NF-κB信号通路.
将NF-κB转移到核中导致与LHR促进器区域结合,抑制LHR转录.
这种转录抑制最终抑制了的合成和分泌.

结论:

PS-MPs通过破坏丸间隙内的细胞间通信来干扰男性生殖功能.
这项研究揭示了一种新的途径,涉及巨细胞衍生TNF-α和NF-κB信号传递,导致LHR下调和降低丸激素的产生.
这些发现为了解PS-MPs对男性生育能力的不良影响提供了分子基础.

关键词:

对于LHR来说,这是非常重要的.巨细胞是一个巨细胞.男性的生殖功能障碍是男性的生殖功能障碍.在 NF-κBB 中.公众党的议员.测试激素测试激素是什么

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