删除维生素D受体在异常机械刺激下加剧了关节病理变化
Tianyi Tang1, Tong Lu2, Baochao Li2
1Department of Orthodontics, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, China; Division of Biomaterials and Tissue Engineering, Eastman Dental Institute, University College London, London NW3 2PF, UK.
Life sciences
|July 14, 2024
概括
维生素D (VD) 和它的受体 (VDR) 对关节 (TMJ) 的健康至关重要. 缺少VDR会使关节炎症恶化,阻碍软骨生长,并在机械压力下增加骨损失.
科学领域:
- 生物医学科学 生物医学科学
- 矯正牙科 矯正牙科是一種矯正牙科.
- 炎症研究 炎症研究
背景情况:
- 关节疾病 (TMD) 涉及无菌性炎症和关节 (TMJ) 的退行性变化.
- 维生素D (VD) 在平衡和免疫调节中起作用.
- 维生素D受体 (VDR) 参与调节炎症反应.
研究的目的:
- 为了研究VD-VDR信号轴在关节病变性退化中的抗炎作用.
- 阐明VD-VDR在机械应力下对关节的影响背后的机制.
主要方法:
- 使用了Vdr-ablated (Vdr-/-res) 鼠标和野生类型 (WT) 控制器.
- 使用单边前部交叉咬伤 (UAC) 模型与机械刺激诱导的关节障碍.
- 进行了组织学,免疫组织化学和微型CT分析.
- 在压力压力下对WT和Vdr-/-红细胞进行了体外研究.
主要成果:
- 在机械应力下,Vdr-/-res小鼠的关节软骨厚度降低,骨质细胞活性增加.
- 在体外,Vdr-/-冠状细胞表现出抑制的增殖和炎症标志物的表达增加 (Il1b,Mmp3,Mmp13).
- 在应对压力的过程中,WT红细胞对TNFα进行了上调调节,这与Vdr-/-红细胞不同.
结论:
- VD-VDR轴对于保持TMJ完整性来应对机械应力至关重要.
- VDR缺乏会加剧关节炎症 (不包括TNFα),损害胆细胞的增殖,并促进骨质再吸收.
- 准VD-VDR信号可能为TMD提供治疗潜力.
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