CircPGM5通过MiR-21-5p/MAPK10轴调节Foxo3a酸化,以抑制膀癌的进展
Chao Cheng1, Ze Zhang2, Jiawei Wang2
1Department of Urology, The Fifth Affiliated Hospital Sun Yat-sen University, Zhuhai, 519000, Guangdong, PR China; Department of Urology, The First Affiliated Hospital, Yijishan Hospital of Wannan Medical College, Wuhu, 241001, Anhui, China; Department of Urology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Suzhou, Jiangsu 215008, PR China.
Cellular signalling
|July 14, 2024
概括
循环RNA PGM5 (circPGM5) 在膀癌 (BC) 中起到瘤抑制作用. 较低的circPGM5水平与晚期BC相关,其恢复通过调节miR-21-5p/MAPK10/Foxo3a通路来抑制癌症进展.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 膀癌 (BC) 是一种普遍存在的恶性瘤,特别是在男性中.
- 循环RNAs (circRNAs) 在BC进展中发挥作用,但它们的机制需要进一步研究.
- 识别新型circRNA及其功能对于理解BC病变的产生至关重要.
研究的目的:
- 在膀癌中识别和表征一种新型circRNA,circPGM5.
- 调查BC.中circPGM5的功能作用和潜在机制.
- 探索circPGM5作为BC的潜在治疗点.
主要方法:
- 高通量测序用于选新型circRNAs.
- 定量实时PCR (qRT-PCR) 用于评估BC组织和细胞系中的circPGM5表达水平.
- RNA光在位杂交 (FISH) 来确定亚细胞局部化.
- 在体外测试 (增殖,迁移,入侵) 和体内瘤模型来评估功能影响.
- 涉及微RNA (miRNA) 相互作用和下游信号通路分析的机制研究.
主要成果:
- 发现circPGM5在BC组织和细胞系中显著下调.
- 在BC患者中,较低的circPGM5表达与高级阶段,级别和淋巴转移相关.
- 过度表达circPGM5抑制BC细胞的增殖,迁移和入侵在体外,并抑制瘤生长和转移在体内.
- 在机械上,circPGM5作为miR-21-5p的海绵,对MAPK10的表达进行上调,随后影响Foxo3a酸化.
结论:
- 在膀癌中,circPGM5作为瘤抑制剂起作用.
- 这种circPGM5/miR-21-5p/MAPK10/Foxo3a轴代表了BC的新型调节途径.
- circPGM5具有作为诊断生物标志物和膀癌治疗点的潜力.
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