基于piperine的纳米乳液对Candida spp.的抗真菌活性 通过在体外苏微稀释试验
Diajeng Sekar Adisuri1, Priya Madhavan1, Pei Pei Chong2
1School of Medicine, Taylor's University Lakeside Campus, Subang Jaya 47500, Malaysia; Digital Health and Medical Advancement Impact Lab, Taylor's University, No.1, Jalan Taylor's. Subang Jaya, 47500 Selangor, Malaysia.
Journal of pharmaceutical sciences
|July 14, 2024
概括
这项研究开发了一种稳定的piperine装载纳米乳液来对抗Candida感染,与纯piperine相比显示出增强的抗真菌活性. 这为阿醇抗真菌药物提供了有前途的替代品,因为其有效性增加和抗性潜力降低.
科学领域:
- 制药科学 制药科学
- 菌类学 菌类学是指菌类学.
- 纳米技术 纳米技术
背景情况:
- кандидемия每年影响75万人,死亡率高,特别是来自Candida glabrata.
- 醇抗真菌药物是常见的治疗方法,但由于副作用和新出现的耐药性而面临限制.
- 皮佩林是一种天然化合物,具有抗真菌潜力,但溶解性差,阻碍了其临床应用.
研究的目的:
- 制定一个稳定的piperine装载的油在水 (O/W) 纳米乳液,以克服piperine的不溶性.
- 为了评估piperine纳米乳液对Candida物种的增强抗真菌功效.
- 为候选血病提供可行的替代治疗策略.
主要方法:
- 使用Cremophor RH40,Transcutol HP和Capryol 90制备一个O/W纳米乳液.
- 使用zetasizer,FTIR和TEM,对纳米乳液特性 (滴滴大小,PDI,泽塔潜力) 的表征.
- 通过 in vitro 汁微稀释试验来确定最小抑制度 (MIC90).
主要成果:
- 成功制备了一种稳定的纳米乳液,滴粒大小为24.37nm,PDI为0.453,泽塔电位为-21.10mV.
- 热稳定性测试显示没有相分离,FTIR/TEM证实了piperine的存在和纳米乳液结构.
- 皮佩林纳米乳液的MIC90值比纯皮佩林低20-50%,表明抗真菌活性显著增强.
结论:
- 皮佩林可以有效地制成一个稳定的纳米乳液.
- 纳米乳液配方显著改善了皮佩林对Candida物种的抗真菌功效.
- 这种piperine纳米乳液代表了治疗候选血症的有希望的替代方案,解决了当前治疗方法的局限性.
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