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在光功酸研究中应用矩阵辅助激光脱/电离

Irina V Minenkova¹, Viacheslav V Lebedev¹, Alexey K Buryak¹

¹The Institute of Physical Chemistry and Electrochemistry RAS (IPCE RAS), Moscow, Russia.

Rapid communications in mass spectrometry : RCM

|July 14, 2024

概括

这项研究使用矩阵辅助激光脱/电离 (MALDI) 来实验分析酸 (PTA) 的结构. 研究人员首次成功识别了PTA二元体,进步了对异聚酸碎片化的理解.

科学领域:

分析化学分析化学
材料科学材料科学材料科学
物理化学物理化学

背景情况:

酸 (PTA) 在催化过程中至关重要,但它的结构主要是理论上研究.
矩阵辅助激光脱吸/电离 (MALDI) 提供了实验性异构酸分析的潜力.
在MALDI的挑战包括高粒子质量和复杂的同位素分布,通过自动信号识别解决.

研究的目的:

通过实验研究酸的结构和碎片化 (PTA).
克服MALDI用于分析高分子量异聚酸的局限性.
开发和应用用于识别质谱信号及其同位素分布的自动化方法.

主要方法:

酸被分析使用激光分解/电离三种不同的矩阵:2,5-二基酸,α--4-基酸,和酸 (SA).
公司内部使用软件通过生成假设分子公式和估计同位素分布来自动识别质谱峰值.
酸 (SA) 被证明是获得信息质谱的最有效矩阵.

主要成果:

使用SA作为矩阵可以首次检测含有PTA二聚体的颗粒.
自动分析确定了PTA二分体颗粒的组成,包括[H3PW12O40]2·2H2O (m/z 5791.2 Da) 和[H3PW12O40]2·4H2O (m/z 5836.5 Da).
其他观察到的物种包括 (WO3) n·PO3-,HPO2·(WO3) n和WO2·(WO3) n集群,后者在混合氧化状态中呈现.

结论:

MALDI和自动识别的结合为PTA结构和碎片化提供了重要的实验洞察力.
这项研究标志着第一份关于含有酸二聚体的颗粒的报告.
开发的自动化程序有效地解决了分析复杂的异聚酸质谱的挑战.

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