聚基的定义折叠模式支持固有的编码生物信息的能力
Nickolas Kankia1, Levan Lomidze2, Skylar Stevenson1
1Department of Chemistry and Biochemistry, Center for RNA biology, The Ohio State University, Columbus, Ohio, USA.
Biopolymers
|July 15, 2024
概括
四重复 (G4) 世界假设表明RNA和DNA可以形成稳定的G-四重复结构. 这项研究表明,RNA G-四复合体与DNA类似.
科学领域:
- 生命的起源研究研究生命的起源.
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 虽然RNA世界假设具有影响力,但在解释早期生命起源方面面临挑战.
- 四重复 (G4) 世界假设提出了多个 (dG) 形式的稳定G-四边形架构,提供了一个无悖论的替代方案.
- 关键问题仍然是关于RNA形成G-四重复的能力及其向沃森-克里克互补性演变的关键问题.
研究的目的:
- 为了调查RNA是否与DNA具有相同的G-四重复折叠模式.
- 探索稳定的G-四复合体如何演变为当前的信息传输系统.
- 分析DNA和RNAG15/G3T序列的热力学和光学特性.
主要方法:
- 热力学和光学性质的系统研究.
- 来自G15和G3T动机的DNA和RNA序列的分析.
- 将DNA和RNA之间的四重复形成和稳定性进行比较.
主要成果:
- 无论是DNA还是RNA序列,都采用稳定的G-四重复结构,具有三个G-四边形和单个G循环.
- 聚 (dG) 和聚 (rG) 具有固有的折叠到定义的3D四重体架构的能力.
- 单核酸替代物显著破坏了DNA和RNAG四重复的稳定性,减少了40°C左右的热稳定性.
结论:
- 像DNA一样,RNA具有形成稳定的G-四重复结构的内在能力,支持G4世界假设.
- 这些四重体对突变的脆弱性为引入互补性提供了潜在的机制.
- 这项研究提供了对早期分子进化的见解,以及从基于G-quadruplex的系统向现代遗传信息传输的过渡.
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