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原细胞对RNA折叠,功能和进化的影响

Ranajay Saha¹, Jongseok A Choi¹, Irene A Chen¹

¹Department of Chemical and Biomolecular Engineering, Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, California 90095-1592, United States.

Accounts of chemical research

|July 15, 2024

概括

在囊泡中封装RNA可以增强 ribozyme 折叠和活性,通过偏好更高性能变体来加速进化. 这一发现提供了关于生命起源和最小细胞的发展的见解.

科学领域:

生命的起源研究生命的起源研究
生物物理学的生物物理.
生物化学生物化学

背景情况:

益生菌是一种前生物.
世界RNARNA世界RNA世界
这一假设表明,RNA在DNA和蛋白质之前起到了遗传和催化作用.
合作系统,就像早期生命一样,需要分隔才能生存和进化.
最小的细胞可能起源于含有RNA代谢的简单囊泡.

研究的目的:

研究膜囊泡内封装对RNA折叠和活性的影响.
探索物理限制如何影响 ribozyme 功能和进化潜力.
了解模型原细胞系统中出现的行为.

主要方法:

使用Förster共振能量转移 (FRET) 来描述里波酶折叠和囊泡内的活性.
采用高通量测序试验来测量众多 ribozyme 变体的氨基化动力学.
进行了体外进化实验以观察适应率.

主要成果:

封装通常通过排除体积效应促进RNA折叠,独立于化学相互作用.
囊泡封闭增加了 ribozyme 的活性,并挽救了突变的 ribozymes 的功能.
封装优先增强高活性 ribozyme 变体,导致更快的进化适应.

结论:

在囊泡中的简单封装可以显著改变RNA的进化格局.
分隔的物理效应,如被排除的体积,对于原细胞的功能和进化至关重要.
研究最小的原细胞为理解从无生命物质到生物系统的过渡提供了一条途径.

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