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Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

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Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
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Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

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The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
726
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
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Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
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Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
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一个逐步的表面电离方法用于离子移动性光谱学.

Jianhua Lin1,2, Xiaoguang Gao1, Jian Jia1

  • 1State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing, China.

Rapid communications in mass spectrometry : RCM
|July 15, 2024
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种新的表面电离-离子移动性光谱法,用于检测呼吸中的三甲基胺. 该技术具有高灵敏度和耐湿性,有望用于早期发现疾病.

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科学领域:

  • 分析化学 分析化学
  • 生物医学工程 生物医学工程
  • 呼吸系统医学 呼吸系统医学

背景情况:

  • 早期发现疾病,如病,可以通过监测呼出的呼吸中的有机化合物来实现.
  • 在复杂的,潮湿的呼出的呼吸中检测微量疾病标志物存在重大分析挑战.
  • 表面电离 (SI) 提供有机化合物的选择性电离,使其适合使用离子移动谱仪 (IMS) 进行呼吸分析.

研究的目的:

  • 开发和评估一种新的逐步表面电离 (SI) 方法,用于检测呼气中的有机化合物.
  • 评估SI-IMS系统在不同度和湿度水平下检测三甲基胺 (TMA) 的性能.
  • 确定SI-IMS的可行性,作为疾病诊断中呼吸分析的敏感和强大的技术.

主要方法:

  • 开发了一种涉及低温吸附和高温电离的逐步SI方法.
  • 三甲基胺 (TMA) 被用作目标分析物,并使用与SI系统相结合的离子运动谱仪检测.
  • 该方法的性能通过分析各种度和湿度水平的TMA来评估,包括人类呼吸中的尖端.

主要成果:

  • 使用SI-IMS方法,成功检测出TMA的度在2至200ppb之间.
  • 在TMA度和峰值离子强度之间观察到强烈的线性相关性 (R2=0.996).
  • 该方法表现出极好的可重复性 (标准偏差<0.306%),在高湿度 (>93%RH,2.7%) 中信号变化最小,以及高恢复率 (106.8%).

结论:

  • 开发的阶段性SI-IMS方法的特点是低电离温度,高灵敏度和强大的耐湿性.
  • 该技术表现出极好的可重复性,使其可靠用于定量分析.
  • SI-IMS是一种有前途的方法,用于用于早期疾病诊断的呼气中的有机化合物的敏感和特定检测.