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Updated: May 2, 2026

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

在基于DNA甲基化的工具中反映的Infinium MethylationEPIC v2.0和v1.0之间的读数差异:对人类人口表观遗传学研究的含义和考虑

Beryl C Zhuang^1,2, Marcia Smiti Jude^1,2, Chaini Konwar^1,2

¹BC Children's Hospital Research Institute, 950 West 28th Avenue, Vancouver, BC, V5Z 4H4, Canada.

bioRxiv : the preprint server for biology

|July 15, 2024

概括

新的Illumina MethylationEPIC BeadChip Infinium微阵列v2.0 (EPICv2) 显示了高阵列级相关性,但与EPICv1.1的可变探针级一致性. 版本差异对DNA甲基化工具产生影响,需要在研究中仔细协调.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
基因组学就是基因组学.
生物技术是生物技术.

背景情况:

伊卢米纳甲基化EPIC BeadChip Infinium微阵列v2.0 (EPICv2) 是一个新的DNA甲基化分析平台.
它与前一个版本 (EPICv1) 的一致性在数组级别很高,但尚未完全评估下游基于DNA甲基化的工具.

研究的目的:

调查EPICv1和EPICv2之间的一致性,用于基于DNA甲基化的工具,包括细胞类型解,表观遗传时钟和生物标志物.
评估EPIC版本差异对这些工具的影响,并探索缓解策略.

主要方法:

用EPICv1和EPICv2对来自四个成年队伍的匹配静脉血液样本进行了DNA甲基化分析.
数据分析侧重于数组级和探针级一致性,以及版本差异对各种基于DNA甲基化的工具的影响.
评估了标准化方法和版本调整策略.

主要成果:

根据EPIC版本分组的样本,表明平台之间的系统差异.
观察到高数组级一致性,但探针级一致性差异很大.
基于DNA甲基化工具的估计显示出显著的版本特定差异,即使在正常化后.

结论:

考虑EPIC版本对于研究至关重要,特别是在元分析和纵向研究中,这些研究涉及不同版本的样本.
版本差异可能会影响全表观基因组关联研究 (EWAS) 的发现,特别是对于不太一致的探针.
根据EPIC版本进行调整或单独分析每个版本的数据可以减轻不一致.

关键词:

通过DNA甲基化.表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.在Illumina的EPIC阵列中,细胞类型的解解.遗传表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表观表炎症和生活方式生物标志物.复制探头进行复制.

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