表观遗传变化调节了人体热囊细胞分化中的表皮细胞-介质细胞过渡
William E Ackerman1, Mauricio M Rigo2, Sonia C DaSilva-Arnold3
1Department of Obstetrics and Gynecology and AI.Health4All Center for Health Equity Using Machine Learning and Artificial Intelligence, University of Illinois College of Medicine, Chicago, USA.
bioRxiv : the preprint server for biology
|July 15, 2024
概括
在怀孕期间,人类胎盘外性热囊细胞 (EVT) 经历了上皮-介质细胞过渡 (EMT). DNA甲基化变化和EMT基因调节对于EVT的分化和入侵至关重要,影响产科疾病.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 人类胎盘外性热囊细胞 (EVT) 差异化涉及增殖和侵入能力的变化.
- 异常的EVT入侵与子宫前和胎盘积分谱系障碍有关.
- 了解正常的EVT差异化对于解决胎盘病理至关重要.
研究的目的:
- 描述人类细胞细胞 (CTB) 到EVT分化期间的基因表达和DNA甲基化变化.
- 为了识别特细胞特异性上皮层-介质细胞过渡 (EMT) 签名.
- 调查表观遗传调节基因在EVT入侵中的功能作用.
主要方法:
- 纯化人类CTB和EVT细胞基因表达分析.
- 全基因组的DNA甲基化概况.
- 使用JEG3 trofhoblast细胞和RUNX1操纵的功能研究.
主要成果:
- 从CTB到EVT的差异化涉及一个特洛细胞特异性的EMT.
- EVT细胞表现出全基因组低甲基化与特定位点甲基化 (GOM) 的增长.
- RUNX1,一个关键的EMT基因,显示GOM,并且对热囊细胞迁移和入侵具有功能重要.
结论:
- 表观遗传调节,特别是特定于位点的GOM,在EVT分化和入侵中起着重要作用.
- 像RUNX1这样的EMT通路和基因对于正常的EVT功能至关重要.
- 这些表观遗传控制过程的失调对产科疾病有影响.
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