在中央代谢酶的进化过程中,自我组装的频繁过渡
Franziska L Sendker1, Tabea Schlotthauer1, Christopher-Nils Mais2
1Max-Planck-Institute for Terrestrial Microbiology; Karl-von-Frisch-Str. 10, 35043 Marburg, Germany.
bioRxiv : the preprint server for biology
|July 15, 2024
概括
酶组合结构可以在进化过程中中性地改变,并不总是为了功能优势. 这项研究揭示了酸盐合成酶中的多种组装类型,显示了蛋白质复合体进化的灵活性.
科学领域:
- 生物化学 生化学
- 进化生物学 进化生物学
- 结构生物学 结构生物学
背景情况:
- 酶通常形成同质复合体,其结构被认为决定功能.
- 这些组合的进化驱动因素并不总是很清楚,一些四级结构在物种之间有所不同.
- 已经提出了蛋白质自我组合的中性变异,但很难在规模上进行研究.
研究的目的:
- 为了研究 prokaryotic 酸盐合成酶中 homo-oligomeric 组合的进化动态.
- 为了确定四级结构的变化是否会影响酶的功能.
- 为了探索蛋白质组合,进化和功能在整个酶类遗传学之间的关系.
主要方法:
- 利用质量摄影仪进行大规模的四分制结构测量.
- 运用了遗传学来分析进化关系.
- 应用结构生物学技术进行详细分析.
- 进行了体外和体内活体功能实验.
主要成果:
- 在酸盐合成酶的整个进化历史上发现了各种各样的同类寡合组装类型.
- 观察到并行进化的实例和组装复杂性的反转.
- 发现不同组件之间的进化过渡不会显著影响酶催化活性.
- 证明了酶可以在没有强大的功能约束的情况下探索各种组装状态.
结论:
- 像酸盐合成酶这样的酶中的同类寡合体组件可以在独立于功能必要性的中立地演变.
- 蛋白质四级结构表现出显著的进化可塑性.
- 描述整个族系的结构-功能关系对于理解蛋白质进化至关重要.
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