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限制硫氨基酸可以通过促进肌肉中的脂肪氧化来提高小鼠的运动能力
Charlotte G Mann1, Michael R MacArthur2,3,4, Jing Zhang1
1Department of Health Sciences and Technology, ETH Zurich, Zurich 8092, Switzerland.
bioRxiv : the preprint server for biology
|July 15, 2024
概括
限制含硫氨基酸 (SAAR) 的饮食可以通过增加脂肪燃烧来提高小鼠的运动能力. 这种代谢转变增强了耐力,表明SAAR是改善身体表现的潜在策略.
科学领域:
- 代谢调节 代谢调节 代谢调节
- 运动生理学 运动生理学
- 营养生物化学 营养生物化学
背景情况:
- 已知含硫氨基酸 (SAAR) 的饮食限制可以改善代谢健康并延长寿命.
- 沙尔对骨肌功能和运动表现的影响尚不清楚.
- 耐力运动与SAAR分享了一些好处,促使对它们的相互作用进行了调查.
研究的目的:
- 研究SAAR对小鼠骨肌肉和运动能力的影响.
- 阐明SAAR诱导的耐力改善背后的代谢和分子机制.
- 为了确定关键的分子参与者,如CD36和VEGF信号传递,在调解这些效应.
主要方法:
- 在年轻的,久坐不动的雄性小鼠中,一周的SAAR.
- 间接热量计用于评估运动期间的代谢率和基质利用率.
- 糖溶性肌肉的转录分析.
- 测量脂肪酸循环流量和肌肉β-氧化.
- 基因操纵 (内皮细胞特异性CD36删除) 和药理抑制 (VEGF信号传递).
主要成果:
- SAAR显著增加了耐力运动能力.
- 沙尔增强了休息时的脂质氧化,并在运动期间延迟了碳水化合物使用.
- 转录组数据显示,肌肉中的脂肪酸代谢基因的表达增加.
- 观察到脂肪酸循环增加和肌肉β-氧化.
- 内皮细胞CD36的删除减弱了SAAR的运动益处.
- 抑制VEGF信号阻断了SAAR诱导的运动改善,但没有影响血管生成.
结论:
- 通过促进骨肌肉中的脂肪酸利用,SAAR提高了小鼠的运动能力.
- 内皮细胞CD36和非正规的VEGF信号对SAAR对运动表现的影响至关重要.
- 萨尔通过涉及内皮脂肪酸运输的机制影响肌肉基质的可用性.
- 这些发现揭示了硫氨基酸代谢,内皮功能和运动适应性之间的新联系.
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