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一个空间分辨的多原子单细胞大豆发育地图

Xuan Zhang^1,2, Ziliang Luo^1,2, Alexandre P Marand^3,2

¹Department of Genetics, University of Georgia, Athens, GA, USA.

bioRxiv : the preprint server for biology

|July 15, 2024

概括

这项研究绘制了大豆组织中的基因调节图,揭示了细胞特异性的调节元素和转录因子. 它为了解不同大豆细胞类型的基因控制提供了基础.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
细胞生物学细胞生物学

背景情况:

基因调节元件 (CREs) 对于精确的基因表达控制至关重要.
了解CREs是解读细胞身份和功能的关键.

研究的目的:

在大豆中创建一个空间分辨的基因表达和染色质可访问性的单细胞地图.
为了识别特定于细胞类型的CREs和转录因子 (TF) 动机.
探索大豆发育和共生中的基因调节网络.

主要方法:

在十个大豆组织中进行单细胞RNA测序和ATAC-seq.
细胞类型的识别,可访问的染色体区域 (ACR) 和TF图案.
发展轨迹的分析和监管网络的保护.

主要成果:

确定了103种不同的大豆细胞类型和303,199个ACR.
发现近40%的ACR是细胞类型特定的,并为定义TF动机而进行丰富.
揭示了内体发育,糖糖运输和胚胎细胞规范中的调节机制,包括用于骨干细胞身份的家庭盒TF.

结论:

这本地图集为研究大豆基因调节提供了全面的资源.
确定了对大豆细胞身份和发育至关重要的新型CREs和TFs.
提供了关于豆类共生固定和作物改善的遗传基础的见解.

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