内皮Zmiz1在视网膜发育过程中调节生理和病理生理血管生成
Nehal R Patel1, K C Rajan1, Mark Y Chiang2
1Department of Cell and Molecular Biology, Tulane University, New Orleans, LA, United States.
bioRxiv : the preprint server for biology
|July 15, 2024
概括
转录辅因子Zmiz1对于血管形成至关重要. 它的缺失会损害胚胎发育,视网膜血管生成和视网膜病变模型中的病理血管生成.
科学领域:
- 血管生物学 血管生物学
- 分子生物学分子生物学
- 发展生物学 发展生物学
背景情况:
- 血管新生是由转录因子调节的,但是辅助因子的作用不太清楚.
- Zmiz1是一种转录辅因子,在内皮细胞中起着未知的作用.
研究的目的:
- 研究Zmiz1在内皮细胞在生理和病理血管生成过程中的作用.
主要方法:
- 在胚胎发育期间和出生后,小鼠的内皮细胞特异性Zmiz1的删除.
- 在小鼠中的氧气诱导视网膜病变 (OIR) 模型.
- 纤维素珠测量. 纤维素珠测量.
- 在体外和体外迁移测定.
- 基因表达分析.
主要成果:
- 内皮Zmiz1删除导致由于血管缺陷而导致胚胎死亡.
- 产后Zmiz1切除损害了视网膜血管生成,减少了血管外生和密度.
- 缺少Zmiz1减少了血管发芽和内皮细胞迁移,降低了尖端细胞基因的调节.
- 在OIR模型中,Zmiz1对于重血管化至关重要.
结论:
- 内皮Zmiz1对于胚胎血管发育,产后视网膜血管生成和病理血管生成至关重要.
- Zmiz1 调节内皮细胞迁移和尖端细胞基因表达.
- Zmiz1在生理和病理血管生成中起着至关重要的作用.
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