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在自由行为小鼠中使用小型化微摄像机阵列显微镜 (mini-MCAM) 进行泛皮层细胞成像.

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    科学领域:

    • 神经科学是一个神经科学.
    • 生物成像是一种生物成像.
    • 大脑皮层研究研究 大脑皮层研究

    背景情况:

    • 了解复杂的行为需要同时从自由移动的动物中分布式的大脑电路记录神经活动.
    • 现有的小型化显微镜的视野有限,阻碍了多区域的神经活动测量.

    研究的目的:

    • 推出一种新的小型化微摄像机阵列显微镜 (mini-MCAM) 用于广域神经记录.
    • 为了在小鼠体内同时进行全皮质,全细胞分辨率的体内成像.

    主要方法:

    • 开发小型化微摄像机阵列显微镜 (mini-MCAM) 配有四个光成像微摄像机.
    • 每个摄像头都在4.5mm x 2.55mm的视野中捕捉神经活动.
    • 在体内 (Ca2+) 图像成像的演示,在头部固定和自由行为小鼠.

    主要成果:

    • 迷你MCAM累积成像超过30mm2的背部皮质.
    • 它捕获来自运动皮层,体感皮层,视觉皮层,后皮层和关联皮层中稀少表达的GCaMP6s神经元的神经活动.
    • 在体内成像中实现了整个皮层的细胞分辨率.

    结论:

    • 迷你MCAM克服了当前用于大规模神经记录的显微镜的FOV限制.
    • 这项技术有助于研究大脑电路协调,从而研究动物的复杂行为.
    • 能够对整个皮层的神经动态产生前所未有的洞察力.