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对共占用N-和O-糖的量化和特定地点的分析

Joann Chongsaritsinsuk¹, Valentina Rangel-Angarita¹, Keira E Mahoney¹

¹Department of Chemistry, Yale University, New Haven, CT.

bioRxiv : the preprint server for biology

|July 15, 2024

概括

这项研究表明,粘膜经常具有N-和O-链 glycosylation (共同占用),需要基于电子的碎片化方法进行准确的分析. 其他葡萄糖蛋白通常只需要碰撞方法.

科学领域:

葡萄糖蛋白质组学
后翻译修改后的修改.

背景情况:

蛋白质糖化,一个关键的翻译后修改,被广泛分类为N-或O-链.
对葡萄糖的特定位点分析依赖于各种碎片化技术,受葡萄糖化类型和可用的仪器仪表的影响.
碰撞解离方法是N-glycoproteomics的常见方法,假设每个的单个N-glycosylation位点,而基于电子的方法对O-glycosite定位至关重要.

研究的目的:

调查粘膜和其他糖蛋白中同时发生的N-和O相关的糖化 (共同占用) 的患病率.
确定当共占有存在时,以电子为基础的碎片化方法对N-glycoproteomics的必要性.
描述O-甘氨酸相对于N-甘氨基化位点的局部和结构.

主要方法:

在各种生物样本中量化N-和O-糖的流行率,包括重组蛋白,纯化糖蛋白和复杂混合物.
基于电子的碎片化方法的应用,用于对N-和O-糖化的特定部位分析.
在N-序列内分析O-糖位的定位,并根据接近N-糖位的O-糖结构的表征.

主要成果:

与非蛋白糖蛋白相比,蛋白表现出N-和O-糖联合占用的频率显著更高.
当共同占用发生时,O-葡萄糖体通常在N-sequon中的Ser/Thr残留物上被发现.
靠近占有Asn残留物的O-甘氨酸通常是未加工的核心1结构,而更远的O-甘氨酸则显示出更延伸的结构.

结论:

基于电子的碎片化方法对于粘膜的综合性特定位点分析至关重要,因为N-和O-糖的共同占用很普遍.
碰撞解离方法通常适用于分析缺乏显著共占用的糖蛋白中的N-糖化.
这些发现凸显了粘膜中独特的糖化模式,并强调了选择适当的分析技术的重要性.

关键词:

在N-glycosylation的过程中.这是一种O-糖基化.在共同占用中.碎片化的碎片化碎片化的碎片化葡萄糖蛋白质组学物质的使用.粘膜粘膜粘膜粘膜

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