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转移性癌细胞在三维微环境中的机械进化

Karlin Hilai¹, Daniil Grubich¹, Marcus Akrawi¹

¹Department of Biomedical Engineering, Wayne State University, Detroit, MI 48202, USA.

bioRxiv : the preprint server for biology

|July 15, 2024

概括

光学Brillouin显微镜在3D中揭示了癌细胞的独特机械特征. 这一进步显著提高了癌细胞分类的准确性,为检测提供了潜在的新生物标志物.

科学领域:

生物物理学的生物物理.
癌症生物学癌症生物学
医疗成像医学成像

背景情况:

细胞生物力学对于癌症转移和瘤进展至关重要.
当前的研究经常使用2D模型,这些模型可能不会准确地反映3D生理环境.
在3Din vitro模型中探测细胞弹性仍然是一个重大的技术挑战.

研究的目的:

在3D癌症模型中开发和应用一种用于评估细胞弹性的新方法.
为了研究癌症球体的机械性质的纵向变化.
探索生物力学特征对癌症分类和检测的有用性.

主要方法:

长度机械成像使用光学布里卢恩显微镜超过八天的癌症球体.
提取空间分辨和时间演变的机械特征.
机器学习和深度学习算法的应用用于癌细胞分类.

主要成果:

光学Brillouin显微镜提供了3D癌症球体的密集机械映射.
当与机器学习相结合时,提取的机械特征显著提高了癌细胞分类准确度,从74%提高到95%.
一个深度学习管道成功地将癌症与正常球体区分开来,仅使用Brillouin图像.

结论:

空间和时间分辨率的3D机械映射是可行的,使用光学Brillouin显微镜.
从3D模型中获得的生物力学特征显示了提高癌症分类和检测的巨大潜力.
癌细胞的机械特性可以作为癌症诊断的新生物标志物.

关键词:

三维微环境是3D微环境.布里卢恩显微镜的显微镜生物力学生物力学乳腺癌乳腺癌乳腺癌细胞球形球形体转移转移转移转移

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