对抗生素耐药性基因和野生和被关押的喜马拉雅中的移动遗传元素进行比较
Jundie Zhai1,2, You Wang1,2, Boyu Tang1,2
1State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China.
PeerJ
|July 15, 2024
概括
喜马拉雅携带各种抗生素耐药性基因 (ARG). 野生比被养的类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类类
科学领域:
- 环境微生物学环境微生物学
- 基因组学就是基因组学.
- 兽医科学是一门兽医科学.
背景情况:
- 喜马拉雅是青海-西藏高原广泛存在的食尸动物.
- 它们暴露在动物尸体中的病原体和抗生素中.
- 可以作为监测抗生素耐药性基因 (ARG) 的潜在生物哨兵.
研究的目的:
- 在野生和被捕的喜马拉雅中对ARG和移动遗传元素 (MGE) 进行比较研究.
- 描述抗体和识别ARG的细菌宿主.
- 分析野生种群和养殖种群之间的ARG特征的差异及其与肠道微生物的相关性.
主要方法:
- 使用元基因组测序来分析肠道内容.
- 鉴定和量化了抗生素耐药性基因 (ARG) 和移动遗传元素 (MGE).
- 统计分析,包括Procustes分析和共发生网络,用于比较野生和被囚禁群体.
主要成果:
- 喜马拉雅的耐药性包括20种类型中的414种ARG亚型,其中β-乳糖耐药性最为丰富.
- 野生与受养相比,表现出较低的宏类-林科萨米德-斜谱素 (MLS) 耐药基因.
- 在临床和高风险ARGs,MGEs以及野生和被囚禁种群之间的耐药性机制中观察到显著的差异.
结论:
- 喜马拉雅拥有复杂的抗体组,在野生和被囚禁的个体之间具有不同的ARG配置.
- 环境暴露会影响野生的肠道耐药性,特别是MLS耐药性.
- 这项研究提供了类ARG的基础见解,并强调了它们在监测抗菌素耐药性的作用.
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