相关实验视频
Updated: Jun 21, 2025

10:43
Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids
Published on: December 23, 2022
3.3K
对食道状细胞癌中编程细胞死亡的分子洞察力
Min Chen1,2,3,4,5,6,7, Yijun Qi2,3,4,5,6,7, Shenghua Zhang2,3,4,5,6,7
1School of Information Engineering, Henan University of Science and Technology, Luoyang, China.
PeerJ
|July 15, 2024
概括
这项研究确定了与编程细胞死亡 (PCD) 相关的五个关键基因,这些基因对于诊断食道状细胞癌 (ESCC) 至关重要. 这些发现为ESCC提供了新的诊断标记和治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 食道状细胞癌 (ESCC) 是一种具有有限治疗选择的侵袭性癌症.
- 编程细胞死亡 (PCD) 途径是癌症进展的组成部分,并提供潜在的治疗点.
- 了解PCD机制对于开发新型ESCC治疗至关重要.
研究的目的:
- 鉴定和描述与食道状细胞癌 (ESCC) 编程细胞死亡 (PCD) 相关的枢纽基因.
- 评估ESCC识别的枢纽基因的诊断准确性.
- 探索这些枢纽基因在ESCC进展中的监管网络和功能作用.
主要方法:
- 差异表达分析和机器学习算法 (SVM,随机森林,LASSO) 用于在ESCC患者数据中选PCD相关基因中的枢纽基因.
- 使用数据库预测了与枢纽基因的转录因子 (TF) 和微RNA (miRNA) 相互作用.
- CIBERSORT算法分析了枢纽基因和免疫细胞之间的关系.
- 在体外实验验验证了选定的枢纽基因对ESCC细胞的功能影响.
主要成果:
- 在149个PCD相关的差异表达基因 (DEG) 中确定了5个枢纽基因 (INHBA,LRRK2,HSP90AA1,HSPB8,EIF2AK2).
- 使用这些枢纽基因的综合诊断模型实现了高诊断精度 (AUC = 0.997).
- 在ESCC中,INHBA,HSP90AA1和EIF2AK2被过度表达;INHBA倒置抑制了ESCC细胞的迁移和入侵.
结论:
- 该研究确定了ESCC病原和与编程细胞死亡相关的进展中的关键分子参与者.
- 鉴定到的枢纽基因显示出ESCC的高诊断潜力.
- 这些发现为开发ESCC的新诊断和治疗策略提供了基础.
相关概念视频
The Intrinsic Apoptotic Pathway
6.5K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.5K
Mitogens and the Cell Cycle
6.4K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.4K
Metastasis
5.5K
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
5.5K
The Extrinsic Apoptotic Pathway
6.3K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.3K

