"埃尔伦仪":一种低成本的,开源的度仪,用于微生物生长的无样本表型化
João Serôdio1, Alexandra Bastos1, Silja Frankenbach1
1CESAM-Centre for Environmental and Marine Studies and Department of Biology, University of Aveiro, Aveiro, Portugal.
PeerJ
|July 15, 2024
概括
研究人员开发了Erlenmeter,这是一个低成本的,开源的度仪,用于监测批量培养中的微生物生长. 该设备可以进行非侵入式的光密度测量,非常适合研究和教育环境.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 仪器化 仪器化 仪器化
背景情况:
- 监测微生物生长对于研究和教育至关重要.
- 测量微生物生长的传统方法可能是耗时的,昂贵的,并有污染的风险.
- 需要可访问的,低成本的工具来实时监测微生物生长.
研究的目的:
- 介绍Erlenmeter的设计和验证,这是一个新的,低成本的,开源的流度计.
- 为了证明Erlenmeter在没有样本采集的情况下测量标准Erlenmeyer瓶中的微生物生长的能力.
- 为了突出Erlenmeter适用于研究应用和教育目的的适用性.
主要方法:
- 埃尔伦米特是使用廉价,现成的电子元件和3D打印部件构建的.
- 光密度测量直接在Erlenmeyer瓶中的批量培养物上进行.
- 度仪使用模型生物进行了测试:微藻 *Phaeodactylum tricornutum*,细菌 *Escherichia coli* 和酵母 *Saccharomyces cerevisiae*.
主要成果:
- 埃伦米特成功地实时测量了微生物培养物的光学密度.
- 对*P. tricornutum*,*E. coli*和*S. cerevisiae*产生了详细的生长曲线.
- 该设备促进了非侵入性监测,减少了污染风险和消耗品的使用.
结论:
- 埃尔伦米特是监测微生物生长动态的具有成本效益和用户友好的工具.
- 它的设计使其适合于各种研究环境,并有价值用于实践教学微生物群体动态.
- 埃尔伦米特通过为商业仪器提供负担得起的替代品来促进科学研究和教育的可访问性.
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