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甲基甲酸和二甲基甲酸破坏了HepG2细胞中的Sirtuins表达

Ana K Gutiérrez-García^1,2, Daniel A Torres-García¹, Antonio De Leon-Rodriguez¹

¹División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, A.C., Camino a la Presa San José 2055, Col. Lomas 4a Sección, San Luis Potosí, SLP, 78216, México.

Toxicology research

|July 15, 2024

概括

低度的二甲基甲酸 (DEP) 和二甲基甲酸 (DBP) 破坏了HepG2细胞中的Sirtuin基因表达. 这些内分泌干扰剂还增加了活性氧物种 (ROS),并在较高剂量时降低了细胞活力.

科学领域:

毒理学毒理学毒理学
细胞生物学细胞生物学
内分泌学在内分泌学.

背景情况:

酸盐,包括乙 (DEP) 和乙 (DBP),是内分泌干扰剂.
甲酸盐暴露与代谢障碍有关.

研究的目的:

研究DEP和DBP对HepG2细胞活性的影响.
评估DEP和DBP对活性氧物种 (ROS) 生产的影响.
确定HepG2细胞中DEP和DBP对Sirtuins的调节.

主要方法:

在48-72小时内,HepG2细胞被暴露在DEP和DBP (0.1100μg/mL) 中.
使用qRT-PCR量化Sirtuins的基因表达.
通过Western-Blot.com分析了Sirtuins的蛋白质水平.

主要成果:

低度 (0.1微克/毫升) 的DEP和DBP改变了sirtuin基因表达 (Sirt3,Sirt4,Sirt5).
DEP (≥1μg/mL) 降低了Sirt1蛋白,而DBP (100μg/mL) 降低了Sirt3蛋白.
细胞活力在100微克/毫升下降,而ROS产量在10和100微克/毫升上升,对于两个酸盐.

结论:

低剂量的DEP和DBP暴露会对Sirtuin表达产生负面影响.
酸盐可以诱导氧化应激并降低HepG2细胞中的细胞活力.

关键词:

细胞毒性细胞毒性内分泌干扰物干扰内分泌干扰物塑化剂是一种可塑化剂.这里是Sirtuinsins的所在地.异种生物的异种生物.

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