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Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

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相关实验视频

Updated: Jun 21, 2025

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

在Arabidopsis中实现高效多基因编辑的多功能克隆策略

Ziqiang P Li^1,2, Jennifer Huard¹, Emmanuelle M Bayer¹

¹UMR 5200 Laboratoire de Biogenèse Membranaire, CNRS-University of Bordeaux, Villenave d'Ornon, France.

|July 15, 2024

概括

这项研究提出了一种新的CRISPR-Cas9方法,用于高效的多重植物基因组编辑. 这种多功能策略允许同时针对多个具有同步Cas9表达的基因,从而在Arabidopsis中实现精确的基因修饰.

关键词:

阿拉比多普西斯塔利亚纳.克里斯普尔-Cas9是什么意思门户® 克隆技术黄金大门的克隆技术植物基因组编辑植物基因组编辑 sgRNA多重复合可以实现.

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Last Updated: Jun 21, 2025

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Published on: April 19, 2019

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High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds

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科学领域:

植物生物技术植物生物技术
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

CRISPR-Cas9是植物基因组编辑的一个关键工具.
进步允许同时针对多基因和可诱导/特定组织的编辑.
量身定制的基因组修改越来越有可能.

研究的目的:

开发一个优化的,模块化的CRISPR-Cas9策略,用于同时针对多基因.
为了使特定植物细胞 (如卵细胞) 中的同步Cas9表达成为可能.
提供可适应诱导或组织特定编辑的多功能系统.

主要方法:

开发了一种结合Gateway®和金门技术的克隆策略.
创建了一个模块化向量系统,用于多重化单导 RNA (sgRNA) 表达.
使用Cas9表达和sgRNA向的多站点网关®技术组装构造.
利用卵细胞特异性促进剂进行同步的Cas9表达.

主要成果:

实现了高的编辑效率,同时避免了马赛克主义.
证明了对多个基因的同时向 (例如,Arabidopsis中的MCTP3和MCTP4).
成功结合多重化的sgRNA表达与同步的Cas9输送.

结论:

开发的系统为植物基因组编辑提供了一种多功能和高效的方法.
模块化方法简化了生成多基因编辑线的过程.
这一策略有助于在植物中进行精确,量身定制的基因改造.