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阿雷卡坚果诱导的AREG促进口腔上皮细胞的增殖,迁移和EMT
Ming Li1, Zhenying Yuan2, Zhangui Tang1
1Xiangya Stomatological Hospital & Xiangya School of Stomatology, Central South University & Hunan Key Laboratory of Oral Health Research, Changsha, China.
Oral diseases
|July 15, 2024
概括
阿拉卡坚果提取物 (ANE) 通过通过AREG-MAPK通路促进增殖,迁移和上皮-介质细胞过渡 (EMT) 来影响人类口腔角质细胞 (HOK) 细胞.
科学领域:
- 口腔生物学 口腔生物学
- 细胞机制 细胞机制
- 分子信号传递是分子信号传递.
背景情况:
- 阿拉卡坚果提取物 (ANE) 是一种常见的物质,已知具有对口腔健康的影响.
- 了解其对人类口腔角质细胞 (HOK) 细胞的特定作用对于评估口腔癌风险至关重要.
- 参与ANE对HOK细胞作用的分子通路需要进一步阐明.
研究的目的:
- 研究ANE对HOK细胞的生物学影响.
- 阐明潜在的分子机制,专注于基因和蛋白质表达.
- 确定AREG-MAPK信号通路在ANE诱导的细胞变化中的作用.
主要方法:
- 细胞计数套件-8 (CCK-8) 测试细胞的增殖.
- 第二代转录组测序用于基因表达分析.
- 实时PCR,Western blot,伤口愈合和Transwell测试用于基因/蛋白质表达和细胞迁移.
- AREG基因过度表达和淘汰实验.
主要成果:
- ANE显示了对HOK细胞增殖的剂量依赖作用,在500μg/mL时促进,在50 mg/mL时抑制.
- 在HOK细胞中,ANE (2 mg/mL) 促进了上皮-介质细胞转换 (EMT).
- ANE上调了AREG蛋白表达,激活了MAPK信号通路,并增强了HOK细胞的增殖和迁移.
结论:
- ANE促进HOK细胞的增殖,迁移和EMT.
- AREG-MAPK信号通路是ANE对HOK细胞影响的关键媒介.
- 这些发现有助于理解与使用阿雷卡坚果相关的口腔潜在恶性疾病的病原性.
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