假定的内核酶降低了 Nilaparvata lugens 中口服RNA干扰的效率
Yuanyuan Gao1,2,3, Tingwei Cai1,2,3, Chang Yu1,2,3
1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.
Pest management science
|July 15, 2024
概括
一种新发现的酶,NldsRNase,在棕色的植物肠中降解双链RNA (dsRNA),减少RNA干扰 (RNAi) 疗效,当口服时. 这就解释了为什么食dSRNA的效果不如注射来控制昆虫害虫的效果.
科学领域:
- 分子生物学分子生物学
- 昆虫学 昆虫学是一门学科.
- 虫害管理 虫害管理 虫害管理
背景情况:
- 在昆虫中,RNA干扰 (RNAi) 的有效性因传递方法而异.
- 由于肠道核酶,口服双链RNA (dsRNA) 的有效性往往较低.
- 在Nilaparvata lugens中,低口服RNAi效率的特定机制以前是未知的.
研究的目的:
- 为了确定导致尼拉帕尔瓦塔 Lugens 的口服RNAi 疗效降低的因素.
- 阐明特定肠内核酶在dSRNA降解中的作用.
- 为开发改进的基于RNAi的害虫控制策略提供见解.
主要方法:
- 在N. lugens.中对假定DNA/RNA非特异性内核酶基因的全基因组识别.
- 鉴定出基因 (NldsRNase) 在不同的体位和组织中的定量表达分析.
- 在体外dSRNA降解试验中,使用肠道提取物和共同递送RNAi实验进行了测试.
主要成果:
- 一个DNA/RNA非特异性内核酶基因NldsRNase被鉴定出来,在N. lugens nymphs和midguts中得到高度表达.
- 与注射相比,NldsRNase表达水平与养后RNAi效率降低相关.
- 在体外实验证实,NldsRNase降解dRNA,降低其在昆虫肠道中的稳定性.
结论:
- 在N. lugens肠道中NldsRNase的存在和活性是限制口服RNAi效率的关键因素.
- 了解NldsRNase的功能可以澄清dRNA养和注射疗效之间的差异.
- 这些发现对于设计针对N. lugens和其他昆虫害虫的有效RNAi策略至关重要.
更多相关视频
10:14Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
Published on: May 4, 2018
13.8K
08:55RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
Published on: May 29, 2020
7.7K
相关概念视频
02:15Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
01:23RNA Interference
26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
02:30siRNA - Small Interfering RNAs
16.7K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.7K