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在盲人墨西哥洞穴鱼中调节发育基因表达的演变

Julien Leclercq¹, Jorge Torres-Paz¹, Maxime Policarpo¹

¹Paris-Saclay Institute of Neuroscience, CNRS and University Paris-Saclay, 91400 Saclay, France.

Development (Cambridge, England)

|July 15, 2024

概括

基因表达调节的变化,主要是cis调节性修饰,推动了Astyanax mexicanus洞穴鱼的早期发育进化. 这些表达变化,而不是编码序列变化,形成了表面和洞穴适应群体之间的形态差异.

科学领域:

发育生物学发展生物学
进化生物学进化生物学
基因组学就是基因组学.

背景情况:

形态多样化可能是由于基因表达调节或蛋白质编码序列的改变造成的.
阿斯蒂亚纳克斯 (Astyanax mexicanus) 为研究早期发育进化提供了一个模型,因为它的表面居住和盲洞穴适应的形态.

研究的目的:

研究驱动Astyanax mexicanus早期发育进化的机制.
为了确定 cis-regulatory 变化与编码序列变化的相对贡献到 inter-morph 差异.

主要方法:

在表面和洞穴适应的Astyanax mexicanus之间进行比较的转录组分析.
在洞穴x表面杂交物中对等基因表达比率的分析,以确定cis-regulatory与trans-regulatory变化.
探索主眼基因rx3的调节,包括其细胞水平和域大小.

主要成果:

20%的转录组显示了形态之间的差异表达.
鉴定出基因调节性变化是基因表达变异的首要驱动因素.
细胞rx3水平由cis调节控制,而rx3域大小受到Wnt和Bmp信号的影响.

结论:

基因表达调节的变化,特别是 cis 调节性修饰,是塑造洞穴鱼早期发育进化的主要力量.
发育过程中的基因表达是由不结合的机制和调节模块控制的,导致形态变化.
蛋白质编码序列的变异在Astyanax mexicanus的早期发育进化中起到了很小的作用.

关键词:

关联监管和跨监管 (cis- and trans-regulation) 是一种关联监管和跨监管方式.在Rx3中使用Rx3.胚胎学胚胎学眼睛眼睛眼睛眼睛这是一个F1混合动力.尾巴的尾巴是一个尾巴.

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