表面电荷调节的聚合物纳米乳液用于跨王国生物膜中的卡瓦克罗尔输送
Jungmi Park1, Zhenting Xiang2,3, Yuan Liu2,4
1Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
ACS applied materials & interfaces
|July 15, 2024
概括
装有卡瓦克罗尔的阴离子纳米乳液显示出优越的透和杀死口腔生物膜,有效地消除99.99%的细菌和真菌. 这一突破为治疗具有挑战性的跨王国口腔感染提供了有希望的策略.
科学领域:
- 口腔微生物学 口腔微生物学
- 纳米技术纳米技术
- 提供抗微生物药物的药物.
背景情况:
- 涉及细菌和真菌的跨王国生物膜在口腔感染中普遍存在,并且难以治疗.
- 生物膜中的细胞外聚合物物质 (EPS) 矩阵阻碍了抗菌素的透.
- 卡瓦克罗尔表现出抗菌性质,但需要有效的输送系统.
研究的目的:
- 针对口服生物膜,研究带有卡瓦克罗尔的纳米乳液 (NEs) 具有不同的表面电荷的有效性.
- 为了比较阴离子 (GMT-NE) 和阴离子 (CMT-NE) 纳米乳液在穿透和消除口服生物膜的性能.
- 评估这些NE与口腔粘膜组织的生物相容性.
主要方法:
- 生物可降解的纳米乳液 (NE) 的制造用阴离子 (瓜尼,GMT-NE) 和阴离子 (碳酸盐,CMT-NE) 表面充电.
- 对大小 (∼250 nm) 和泽塔电位 (+25 mV 对于GMT-NE, -33 mV 对于CMT-NE) 的NE的表征.
- 三维光标签和动态跟踪,以评估NE在口腔生物膜中的扩散.
- 在生物膜中对链球菌和*Candida*物种的抗微生物疗效的评估.
- 使用口腔粘膜组织培养物进行生物相容性测试.
主要成果:
- 与CMT-NE相比,GMT-NE在口腔生物膜中的扩散和透率显著更高.
- 在生物膜中,GMT-NE实现了对链球菌和*Candida*物种99.99%的杀死率.
- CMT-NE的疗效较低,大约减少了细菌活力的4log.
- 无论是GMT-NE还是CMT-NE,都与口腔粘膜组织具有良好的生物相容性,没有表现出不良的形态或结构变化.
结论:
- 表面电荷极大地影响了纳米乳液与跨王国口服生物膜的相互作用和对其有效性.
- 带有阴离子卡瓦克罗尔的纳米乳液 (GMT-NE) 是对抗口腔生物膜感染的高度有效策略.
- 这些发现为开发先进的纳米载体提供了宝贵的见解,用于治疗复杂的口腔微生物挑战.
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