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Updated: May 6, 2026

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

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更新:通过注射原始CD4⁺ T细胞 (T细胞转移型大肠炎模型) 在免疫缺陷小鼠中诱导炎症性肠病

Claire F Pearson¹, Kevin J Maloy²

¹Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK.

Current protocols

|July 15, 2024

概括

这项研究详细介绍了一种小鼠模型,通过将特定的T细胞转移到Rag缺乏的小鼠中来研究炎症性肠病 (IBD). 这种模型有助于研究粘膜免疫调节在IBD的发病和治疗.

科学领域:

免疫学免疫学免疫学
胃肠病学胃肠病学
动物模型动物模型

背景情况:

人类的异常性炎症性肠病 (IBD) 呈现出复杂的免疫失调.
T细胞转移模型提供了一种可复制的方法来研究IBD病原体.
淋巴细胞缺乏宿主对于诱导和研究T细胞介导性结肠炎至关重要.

研究的目的:

描述一种强大的方法来诱导Rag缺乏的小鼠的肠道炎症.
为研究目的建立一个模仿人类IBD的模型.
为监测大肠炎的发展和进展提供协议.

主要方法:

采用原始CD4+CD45RBhi T细胞转移到缺乏重组激活基因 (Rag) 的小鼠中.
使用免疫磁性负选择和光激活细胞分类来净化T细胞.
通过减肥,腹和结肠病理学的组织学评估来监测疾病进展.

主要成果:

成功诱导肠道炎症与人类IBD共享特征.
表皮细胞增生,密室形成和淋巴细胞透的证明.
建立一个灵活和可重复的IBD研究模型.

结论:

在Rag缺乏小鼠中的T细胞转移模型是研究IBD的宝贵工具.
这个模型有助于理解IBD中的粘膜免疫调节.
描述的协议有助于对IBD病原和治疗策略进行可复制的研究.

关键词:

CD4+ CD4+ CD4+ CD4+ CD4+ CD4+ CD4+ CD4+ CD4+ CD4+ CD4+转移T细胞转移T细胞大肠炎是一种大肠炎.炎症性肠病是一种炎症性肠病.鼠标模型鼠标模型鼠标模型

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