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MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...

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Updated: Jun 21, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

通过多模式生物传感器进行快速微生物分析,用于横向分析.

Jyong-Huei Lee¹, Siew Mei Chin¹, Dennis C Chan¹

¹Department of Biomedical Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802, United States.

Analytical chemistry

|July 15, 2024

概括

一个新的平台,多模生物传感器用于横向分析 (MBioTA),快速分析微生物群落. 它在短短2小时内确定了分类学,生存能力和功能,推进了微生物组研究和诊断.

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Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

相关实验视频

Last Updated: Jun 21, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

科学领域:

微生物学微生物学
生物技术是生物技术.
主体与微生物的相互作用

背景情况:

传统的微生物分析方法缓慢,缺乏绝对丰度测量,难以检测低生物质,无法区分活细胞/死细胞或分析功能.
了解宿主-微生物相互作用对生物学和医学至关重要,但目前的技术存在重大局限性.

研究的目的:

引入一个快速的多式微生物表征平台,多式生物传感器用于横向分析 (MBioTA).
为了同时捕获微生物群分类,生存能力和功能基因.
通过提供快速,灵敏和全面的微生物分析来克服传统方法的局限性.

主要方法:

开发MBioTA平台,集成单细胞生物传感器,可扩展的微波阵列和自动图像处理.
快速横向分析在短短2小时内完成.
多模式生物传感器同时在单细胞水平上评估分类,生存能力和基因表达.

主要成果:

MBioTA实现了对微生物群组成,生存能力和功能进行快速横向分析.
自动图像处理使细菌的量化能够降至0.0075%的灵敏度,适用于低生物质样本.
平台在家族性阿尔茨海默病小鼠模型中分析肠道微生物群的应用性得到证明.

结论:

MBioTA提供了一种有效,快速和可扩展的解决方案,用于全面的微生物表征.
该平台的功能可以显著推进基础研究和临床诊断.
MBioTA有可能彻底改变与微生物与宿主相互作用相关的疾病的理解和管理.