在CbCyp51中介脱甲基化抑制剂中,耐药性由Codon Bias调节
Lorena I Rangel1,2, Nathan Wyatt1,3, Isaac Courneya1,3
1Sugarbeet Research Unit, Edward T. Schaffer Agricultural Research Center, U.S. Department of Agriculture-Agricultural Research Service, Fargo, ND, U.S.A.
Phytopathology
|July 15, 2024
概括
在Cercospora beticola的真菌杀菌剂耐药性与CbCyp51基因的特定突变有关. 了解这些遗传变化有助于管理甜菜疾病和杀菌剂的应用.
科学领域:
- 植物病理学 植物病理学
- 农业科学 农业科学
- 菌类学 菌类学是指菌类学.
背景情况:
- 由Cercospora beticola引起的Cercospora叶斑是全球对甜菜生产的重大威胁.
- 耐受醇脱甲基化抑制剂 (DMI) 杀菌剂,如四可纳,已与CbCyp51基因的突变有关.
研究的目的:
- 调查CbCyp51中同名和非同名突变之间的关联以及对四种DMI杀菌剂 (四可可纳,prothioconazole,difenoconazole, mefentrifluconazole) 的耐药性.
- 鉴定CbCyp51单元型及其与C. beticola分离物中DMI真菌杀菌剂耐药表型的相关性.
主要方法:
- 从北达科他州和明尼苏达州收集和表型化593个C. beticola分离物,以抵抗四种DMI杀菌剂.
- 鉴定了五种CbCyp51单元型及其与观察到的真菌杀菌剂耐药性表型的关联.
- 定量PCR (qPCR) 用于评估不同单元型的CbCyp51基因表达水平.
主要成果:
- 几乎一半的分离物对所有四种DMI都表现出耐药性,而20%对两个DMI有耐药性,对另外两个DMI敏感.
- 鉴定了五种CbCyp51单元型,其中占主导地位的单元型 (E170_A/L144F_C) 准确预测了对所有四种DMI的耐药性 (准确率为97.6%).
- 第二个最常见的单元型 (E170_A/L144) 与对四可纳和前列可纳的耐药性相关,但对二可纳和美芬特rifluconazole (98.4%准确率) 的敏感性.
- 在单体类型之间没有检测到CbCyp51基因表达的显著差异.
结论:
- CbCyp51中的同义和非同义突变,特别是氨基酸位144和170的突变,与C. beticola. beticola的DMI真菌杀菌剂耐药性密切相关.
- 的使用在真菌杀菌剂耐药性机制中起着重要作用,影响了多种DMI杀菌剂的疗效.
- 研究结果为了解真菌杀菌剂应用策略和糖种植中的耐药性管理提供了关键的见解.
相关概念视频
Genome Copying Errors
4.2K
DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their survival. Therefore, the copying errors are checked and repaired at three levels.
4.2K
Epigenetic Regulation
3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
3.0K
Mismatch Repair
4.8K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
4.8K
Covalently Linked Protein Regulators
6.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
6.8K


