在莲花树根中酸转移酶FcElp3的功能性表征引起腐烂的真菌Fusarium commune
Lifang Ye1,2, Weigang Kuang1,2, Lianhu Zhang1,2
1College of Agronomy, Jiangxi Agricultural University, Nanchang, Jiangxi, 330045, China.
Phytopathology
|July 15, 2024
概括
FcElp3是Fusarium公社中的一个组乙转移酶,对真菌生长,发育和病原性至关重要. 它的删除会影响应激反应,并减少莲花根茎腐烂中的毒性.
科学领域:
- 菌类学 菌类学是指菌类学.
- 植物病理学 植物病理学
- 分子生物学分子生物学
背景情况:
- 菌体共同体是导致莲花根茎腐烂的关键病原体.
- 希斯乙转移酶对于真菌生长和毒性至关重要.
研究的目的:
- 为了研究FcElp3,F.communal中的基酸转移酶在真菌生长和致病性中的作用.
主要方法:
- 一个 ΔFcElp3 突变菌株的构造.
- 细胞生长,胞分泌和应激反应 (奥斯摩斯,细胞壁,氧化) 的分析.
- 在莲花上进行亚细胞局部化,西部涂抹 (H3K14,H4K8乙化),RNA测序和致病性分析.
主要成果:
- 删除FcElp3减少了菌根细胞的生长和化.
- 突变者表现出改变的应激耐受性,增加了对透/细胞壁应激的耐受性,但对氧化应激的敏感性.
- FcElp3对于H3K14和H4K8的乙化至关重要,影响基因表达 (例如Fcap1) 并降低病原性.
结论:
- 在FcElp3的生长,发育,应激反应和病原性方面,FcElp3起着重要作用.
- FcElp3调节多个向基因,包括那些参与氧化应激反应的基因.
- 准FcElp3可能是控制莲花根茎腐烂的策略.
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