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Updated: Jun 21, 2025

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
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反向醇捕获方法,以评估金属结合性线粒体蛋白的醇状态

Hui Zhong1, Eva Nyvltova2, Antoni Barrientos3,4

  • 1Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, FL, USA.

Methods in molecular biology (Clifton, N.J.)
|July 15, 2024
PubMed
概括

二硫化物交换对细胞氧化还原平衡和蛋白质调节至关重要. 一种新的反向醇捕获方法有助于分析包括金属蛋白在内的蛋白质中的氨酸氧化还原状态.

关键词:
美国航空航天管理系统 (AMS)半氨酸二硫化物囊氨酸的氧化方式美国IAA IAA线粒体中的线粒体.转毒生物学 转毒生物学醇的捕获方式

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Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
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