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分子方法提高了检测皮肤相关的菌株 pyogenes 和 emm 类型的分布在儿童中

Jennifer N Hall^1,2,3, Edwin P Armitage^4,5, Elina Senghore⁴

¹Division of Clinical Medicine and National Institute for Health and Care Research Sheffield Biomedical Research Centre, School of Medicine and Population Health, University of Sheffield, Sheffield, United Kingdom.

The Journal of infectious diseases

|July 15, 2024

概括

细菌培养低估了Streptococcus pyogenes在皮肤感染中的作用,特别是在金黄色葡萄球菌. 像qPCR这样的分子方法对于在风湿性心脏病流行地区准确检测至关重要.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
公共卫生公共卫生

背景情况:

链球菌 (S. pyogenes) 的皮肤感染与低资源环境中的风湿性心脏病 (RHD) 有关.
这些感染往往与同时发生.
细菌培养可能低估了S. pyogenes在皮肤炎中真正的流行率.

研究的目的:

为了比较细菌培养和定量聚合酶连锁反应 (qPCR) 的诊断准确性,用于S. pyogenes in pyoderma.
评估S. pyogenes和黄金葡萄球菌联合感染在儿科皮肤炎的流行率.
评估基于PCR的直接 emm-typing用于S. pyogenes菌株的特征.

主要方法:

多重qPCR试验被用于检测S. pyogenes,S. aureus和Sarcoptes scabiei在冈比亚5岁以下儿童的250个皮肤皮样中.
在阳性样本上进行了基于PCR的直接 emm-typing.
结果与以前的细菌培养和全基因组测序 (WGS) 数据进行了比较.

主要成果:

在80%的病变中,qPCR检测到S. pyogenes,明显高于通过培养检测到的51%.
与单独S. pyogenes (95.4%) 相比,S. aureus共感染 (59.9%) 的情况下,对S. pyogenes的培养敏感性降低了.
基于PCR的直接 emm-typing确定了52种S. pyogenes emm-types,其中6种被遗漏的WGS.

结论:

细菌培养显著低估了S. pyogenes在皮肤炎中的负担,特别是当S. aureus存在时.
分子方法对于在RHD流行地区精确检测S. pyogenes至关重要.
加强检测对于监测和评估用于RHD预防的干预措施至关重要.

关键词:

这是一种Streptococcus pyogenes.在EMM-typing中使用.这是一个PCRPCR.皮肤感染皮肤感染菌株多样性菌株多样性

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