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Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
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Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
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细菌外膜囊泡纳米机器人

Songsong Tang1,2, Daitian Tang1,3,4, Houhong Zhou1,5

  • 1Institute of Urology, The Third Affiliated Hospital of Shenzhen University, Shenzhen 518000, People's Republic of China.

Proceedings of the National Academy of Sciences of the United States of America
|July 15, 2024
PubMed
概括
此摘要是机器生成的。

由细菌外膜囊泡 (OMV) 制成的由酶驱动的纳米机器人提供了更好的生物相容性和向瘤治疗. 这些OMV纳米机器人有效地提供基因沉默工具,并增强免疫反应,用于优异的瘤抑制.

关键词:
细菌外膜囊泡是一种细菌.酶推进的推进方式纳米机器人是如何工作的表面生物工程 表面生物工程

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科学领域:

  • 生物医学工程 生物医学工程
  • 纳米技术纳米技术
  • 在瘤学瘤学.

背景情况:

  • 目前的纳米机器人经常使用无机材料,限制了生物相容性和生物功能.
  • 需要先进的纳米机器人系统,以提高效率的精密疗法.
  • 细菌外膜囊泡 (OMVs) 具有内在的生物相容性和多功能表面修饰潜力.

研究的目的:

  • 开发使用细菌外膜囊泡 (OMVs) 进行增强瘤治疗的新型酶驱动纳米机器人.
  • 设计OMV纳米机器人以有针对性地传递小干扰RNA (siRNA) 并改善治疗结果.
  • 评估OMV纳米机器人的实验室和体内瘤抑制疗效.

主要方法:

  • 在OMV膜上固定尿酶以催化纳米机器人推进的尿素分解.
  • 表面生物工程OMV纳米机器人与细胞透化物用于瘤向.
  • 将小干扰RNA (siRNA) 装入OMV中,以防止降解.
  • 在实验室和体内研究使用动物模型,包括正管膀瘤模型.

主要成果:

  • 以酶为动力的OMV纳米机器人展示了有效的推进和增强的瘤向.
  • OMV纳米机器人成功地保护了siRNA免受酶分解,并促进了其传递.
  • 与静态对照组相比,观察到siRNA传递和免疫刺激的显著增强.
  • 在体内实现了实质性的瘤抑制,特别是在正确的膀瘤模型中.

结论:

  • 用酶驱动的OMV纳米机器人代表了先进精确癌症治疗的有希望的平台.
  • 这些纳米机器人提供了更好的生物相容性,有针对性的输送,以及增强的治疗疗效.
  • OMV纳米机器人设计为生物医学应用中多功能和适应性强的医疗机器人打开了新的道路.