由内皮衍生的细胞外囊泡表达细胞间粘附分子反映内皮透性和败血症严重程度
Yusuke Takei1, Mitsuhiro Yamada2, Koji Saito3
1From the Department of Anesthesiology and Perioperative Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan.
Anesthesia and analgesia
|July 15, 2024
概括
表达PECAM或VE-cadherin的内皮衍生的细胞外囊泡 (EDEVs) 显示出作为败血症严重程度和内皮透性的生物标志物的潜力. 这些EDEV的高水平与败血症严重程度的增加相关,具有诊断和预后价值.
科学领域:
- 血管生物学 血管生物学
- 败血症病理生理学病理生理学
- 生物标志物发现发现
背景情况:
- 在败血症中缺乏内皮膜透性的临床指标.
- 内皮衍生的细胞外囊泡 (EDEVs) 是内皮损伤的新兴生物标志物.
- 血小板内皮细胞粘附分子 (PECAM) 和VE-cadherin调节内皮细胞的透性.
研究的目的:
- 调查表达PECAM或VE-cadherin的EDEV与内皮通透性之间的关联.
- 评估这些EDEV作为败血症严重程度生物标志物的潜力.
主要方法:
- 人类静脉内皮细胞 (HUVEC) 用TNF-α刺激,有或没有透性修饰试剂.
- 用德克斯试验和超内皮电阻评估了内皮透气性.
- 在患有败血症 (n=30),败血性休克 (n=42) 和非败血症对照 (n=30) 的患者中,通过流动细胞计量分析了循环中的EDEV水平 (PECAM+,VE-cadherin+).
主要成果:
- 刺激TNF-α增加了HUVEC的透性和EDEV的释放.
- ангиопоетин-1 和 простациклин抑制了 TNF-α 诱导的透性和 EDEV 释放,而 VEGF 则加剧了它.
- 与败血症患者相比,患有败血症休克的患者表现出明显更高的PECAM+和VE-cadherin+EDEV水平,其水平至少在5天内保持升高.
结论:
- 表达PECAM和VE-cadherin的EDEV可以作为增加内皮透性的指标.
- 这些EDEV显示出作为有价值的毒症诊断和预后标记物的希望.
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