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插入与长目标重复在菌素B诱导的沙门氏菌的耐药突变

Tongtong Zhang¹, Huifen Jiang¹, Ying Zhao²

¹Department of Biotechnology and Science, School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, China.

Journal of global antimicrobial resistance

|July 15, 2024

概括

沙门氏菌日记菌株S499通过一个独特的DNA复制事件发展出多素B耐药性. 这导致关键基因的表达增加,导致S499V突变的观察到的抗药性.

科学领域:

微生物学微生物学
基因组学就是基因组学.
抗微生物耐药性抗微生物耐药性

背景情况:

沙门氏菌 (Salmonella enterica) 的一个亚种. 日记虫 (S. diarizonae) 是一个重要的病原体.
聚米辛B是一种关键的抗生素,用于治疗多抗药性格拉姆阴性感染.
了解耐药机制对于有效的治疗策略至关重要.

研究的目的:

调查临床S. diarizonae菌株中多素B耐药性的基因组基础.
识别与获得耐药性相关的遗传变化和基因表达变化.

主要方法:

在S. diarizonae菌株S499中诱导多素B耐药性,以产生突变S499V.
使用Illumina HiSeq X-10和PacBio RS II平台进行全基因组测序.
通过逆转录定量聚合酶链反应 (RT-qPCR) 进行基因表达分析.

主要成果:

在S499中,一个40kb的DNA区域在S499V中进行了三倍化,形成了一个并列重复区域.
重复区域包含 pmrD,pmrG 和 arnBCADTEF 操作子,其旁边是 IS1.
在重复区域内的一些基因中观察到截断突变,pmrD,pmrG和arnT在S499V中显示出显著的上调.

结论:

观察到的DNA重复和随后的pmrD,pmrG和arnt操作子的过度表达可能是S. diarizonae S499V.中的多素B耐药性的原因.
这项研究突出了沙门氏菌获得抗生素耐药性的新机制.

关键词:

过度表达的基因表达过度长DNA片段的重复复制聚米辛b的耐药性聚米辛b的耐药性在沙门氏菌日记中记录.

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