相关实验视频

Updated: May 1, 2026

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

同义词:综合性寡核酸修饰和杂质的综合数据库,以帮助其通过质谱学进行表征

Jennifer L Lippens¹, Biancamaria Florenzi², Katyeny Manuela Da Silva¹

¹Janssen Pharmaceutica NV, Turnhoutseweg 30, 2340 Beerse, Belgium.

Journal of the American Society for Mass Spectrometry

|July 15, 2024

概括

一个新的在线数据库,SynONIM,集中了关于合成寡核酸修饰和杂质的信息. 这种工具有助于质谱分析,这对于生物治疗开发和表征至关重要.

科学领域:

生物制药学分析
质谱测量质量谱测量
橄核酸的化学成分

背景情况:

寡核酸被越来越多地用作生物治疗药物,需要先进的表征.
合成修改增强了寡核酸的有效性和稳定性,但使质谱数据分析复杂化.
杂质是由于修改和压力条件而产生的,这给分析带来了挑战.

研究的目的:

解决对合成寡核酸修饰物和杂质的统一资源的需求.
为基于质谱的寡核酸的特征提供一个集中数据库.
开发一个网络工具来查询关于寡核酸修饰和杂质的信息.

主要方法:

开发一个名为SynONIM的在线数据库.
包括结构,质量和缩写名称的数据,用于修改和杂质.
基于元素组成,质量转移,符号,位置和来源的查询功能的实现.

主要成果:

SynONIM提供了一个全面的,可搜索的合成寡核酸修饰的存储库.
该网络工具有助于高效地检索用于质谱分析的数据.
根据分析开发相关的各种参数组织信息,以便快速访问.

结论:

SynONIM 对于寡核酸分析开发社区来说是一个有价值的资源.
该数据库简化了复杂合成寡核酸的表征.
SynONIM提高了基于质谱学的生物治疗分析的效率和准确性.

更多相关视频

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

相关概念视频

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...