在人类癌症患者和患者衍生模型中进行代谢重编程
Teresa W-M Fan1, Richard M Higashi2, Andrew N Lane2
1Center for Environmental and Systems Biochemistry; Markey Cancer Center; Department of Toxicology and Cancer Biology, University of Kentucky, Lexington, Kentucky 40536, USA twmfan@gmail.com.
Cold Spring Harbor perspectives in medicine
|July 15, 2024
概括
患者衍生有机型组织培养 (PD-OTC) 和有机体 (PDO) 更好地模拟癌症.
科学领域:
- 在瘤学瘤学.
- 代谢学 代谢学 代谢学
- 癌症生物学 癌症生物学
背景情况:
- 瘤微环境 (TME) 显著影响癌症代谢重编程,影响发展和耐药性.
- 准确地建模TME对于了解癌症进展和确定治疗点至关重要.
- 在体内患者研究为评估癌症模型提供了必要的基准.
研究的目的:
- 为了比较患者衍生器官类型组织培养 (PD-OTC) 和患者衍生瘤外移植 (PDTX) 在复制体内癌症代谢重编程中的有效性.
- 评估PD-OTC和患者衍生器官 (PDO) 在研究TME依赖的代谢重编程和治疗反应中的有用性.
- 探索将PD-OTC和PDO模型与单细胞'omics相结合的潜力,以发现新的目标.
主要方法:
- 使用稳定同位素溶解代谢学来分析代谢网络.
- 患者衍生器官型组织培养 (PD-OTC) 和患者衍生瘤外移植 (PDTX) 被用作癌症模型.
- 专门研究了非小细胞肺癌模型.
主要成果:
- 与PDTXs相比,PD-OTCs显示了体内代谢重编程的优越复述.
- PD-OTCs使免疫代谢反应和TME依赖药物耐药性的审讯成为可能.
- PDO还在建模TME依赖的代谢重编程和评估治疗结果方面证明有效.
结论:
- PD-OTC和PDO是研究TME依赖癌症代谢和耐药性的有价值模型.
- 将这些模型与单细胞'omics相结合,为了解人类癌症代谢重编程提供了一种强大的方法.
- 这项研究有助于识别用于癌症治疗和药物耐药性调节的新代谢标.
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