氧化和碳酸应激诱导的AMD和Codonopsis lanceolata通过控制氧化和碳酸应激来改善AMD
Soon-Young Lee1, Yeon-Kyoung Cho2, Chun-Sik Bae3
1College of Korean Medicine, Dongshin University, Naju, 58245, Jeonnam, Korea.
Scientific reports
|July 15, 2024
概括
在与年龄相关的黄斑变性 (AMD) 的体外模型中,Codonopsis lanceolata显示出在预防细胞死亡方面的潜力. 这项研究使用A2E和蓝光建立了AMD模型,发现C.lanceolata可以保护细胞免受损伤.
科学领域:
- 眼科和视觉科学 眼科和视觉科学
- 细胞生物学 细胞生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 与年龄相关的黄斑变性 (AMD) 是不可逆转失明的主要原因.
- 目前对AMD的治疗方法有限,强调需要预防策略和新型治疗剂.
- 开发可靠的体外模型对于研究AMD机制和评估潜在的候选药物至关重要.
研究的目的:
- 建立与年龄相关的黄斑变性 (AMD) 的适当的体外缩模型.
- 为了评估Codonopsis lanceolata的抗AMD作用和治疗机制.
- 为了确定A2E剂量和蓝光强度的最佳条件,用于AMD建模.
主要方法:
- 使用A2E和蓝光处理的ARPE-19细胞创建了体外AMD模型.
- 实验组包括对照组,AMD模型,黄蛋白治疗和不同剂量的Codonopsis lanceolata.
- 通过免疫光学,MTT,TUNEL,FACS和西式涂抹,评估了亡,氧化应激 (Keap1/Nrf2/HO-1) 和碳基应激 (4-HNE).
主要成果:
- 确定30μM A2E和20mW/cm2的蓝光有效地诱导ARPE-19细胞中的缩性AMD模型.
- 在剂量依赖的Codonopsis lanceolata保护ARPE-19细胞免受A2E和蓝光诱导的细胞死亡.
- C.lanceolata通过激活Keap1/Nrf2/HO-1通路,减少4-HNE,并调节Bcl-2家族蛋白质来表现出抗亡作用.
结论:
- 科多诺普西斯兰塞奥拉塔 (Codonopsis lanceolata) 显示出作为治疗与年龄相关的黄斑退化症的治疗剂的显著前景.
- 该研究确定了适合体外AMD模型的特定A2E和蓝光度.
- 兰塞洛塔菌的保护机制包括调节氧化应激和亡途径.
关键词:
科多诺普西斯 (Codonopsis lanceolata) 是一种类植物.在 4-HNE 中,与年龄相关的黄斑变性 (AMD)细胞灭亡 (apoptosis) 是一种死亡的过程.凯普1/Nrf2/HO-1通道中的一个.氧化/碳烯应激作用的压力更多相关视频
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