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相关概念视频

Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

3.9K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
3.9K
The Ras Gene02:38

The Ras Gene

6.2K
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
6.2K
Rab Cascades01:25

Rab Cascades

2.6K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
2.6K
Rab Proteins01:14

Rab Proteins

3.9K
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
3.9K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

5.4K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.4K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

2.7K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.7K

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相关实验视频

Updated: Jun 21, 2025

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
10:27

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Published on: March 9, 2012

10.8K

RAS GTPases和RASSF因子之间的复杂相互作用调节了YAP的亚细胞局部化.

Swati Singh1, Gabriela Bernal Astrain1, Ana Maria Hincapie2

  • 1Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, QC, H3T 1J4, Canada.

EMBO reports
|July 15, 2024
PubMed
概括

这项研究绘制了BRAF,RASSF蛋白和小GTPases之间的相互作用图,揭示了新的效应器功能和信号通路. 这项研究揭示了RASSF蛋白在细胞过程中的新角色,如海马信号传递和线粒体动力学.

关键词:
河马的路径 河马路径镜子 (MIRO) 是一个镜子.这就是RAS GTPase.在RASSF的基础上.哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈

更多相关视频

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
13:51

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay

Published on: November 11, 2018

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Comparing the Affinity of GTPase-binding Proteins using Competition Assays
10:37

Comparing the Affinity of GTPase-binding Proteins using Competition Assays

Published on: October 8, 2015

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相关实验视频

Last Updated: Jun 21, 2025

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
10:27

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Published on: March 9, 2012

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Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
13:51

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay

Published on: November 11, 2018

9.8K
Comparing the Affinity of GTPase-binding Proteins using Competition Assays
10:37

Comparing the Affinity of GTPase-binding Proteins using Competition Assays

Published on: October 8, 2015

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科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 拉斯GTPases是细胞功能的关键调节者,将上游信号转化为下游反应.
  • 效应因子通常通过特定域 (如RBD/RA) 结合GTP结合的GTP酶,但它们在RAS超级家族中的特异性尚不清楚.

研究的目的:

  • 系统地绘制BRAF,RASSF效应器和各种小GTPases (RAS,RHO,ARF) 之间的相互作用.
  • 探索这些相互作用的特异性,并发现新的生物功能.
  • 研究RASSF蛋白在细胞信号传递和器官动态中的作用.

主要方法:

  • 蛋白质与蛋白质相互作用的系统映射.
  • 生物化学试验验证GTPase-effector复合体的有效性.
  • 细胞测试以评估信号通路激活和器官分布.

主要成果:

  • 确定了39个经过验证的GTPase-effector复合体,在RASSF结合中显示出可塑性.
  • BRAF对经典的H/K/NRAS GTPases表现出特定的结合.
  • RASSF5-RAS相互作用激活了Hippo信号和YAP封存.
  • RASSF8形成了与RAS和RHO GTPases相隔的冷凝物.
  • RASSF3被确定为线粒体MIRO蛋白的潜在效应因子,影响有机体分布.

结论:

  • 对GTPase-effector相互作用的系统映射揭示了复杂的结合模式和新的生物功能.
  • 拉斯夫蛋白质扮演着不同的角色,包括调节信号通路和器官动态.
  • 这种方法可以发现由GTPase-effector网络介导的以前未知的细胞过程.