FGF2在来自肺细胞的细胞外囊中分泌
Nelida C Olave1, Brian Halloran1, Namasivayam Ambalavanan1
1Department of Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama, United States.
概括
肺细胞在细胞外囊泡 (EV) 中释放基本纤维细胞生长因子 (FGF2),特别是在高氧化期间. 这些携带FGF2的EV被其他细胞内化,可能会影响肺损伤和修复机制.
科学领域:
- 细胞生物学 细胞生物学
- 肺部医学 肺部医学
- 生物化学 生物化学
背景情况:
- 基本纤维细胞生长因子 (FGF2) 对于细胞生长和修复至关重要,但缺乏用于常规分泌的信号.
- 细胞外囊泡 (EVs) 在肺部中介细胞间通信,并与高氧性肺损伤和支气管肺功能失调有关.
- 了解FGF2出口机制对于肺部疾病研究至关重要.
研究的目的:
- 调查FGF2是否通过不同类型肺细胞的EV分泌.
- 为了确定高氧化对EVsFGF2分泌的影响.
- 在受体细胞内识别FGF2的潜在细胞内结合伙伴.
主要方法:
- 初级人类支气管上皮细胞 (HBE),肺动脉内皮细胞 (HPAE) 和纤维细胞 (HNF) 细胞在正常和高氧条件下培养.
- 使用超离心法将电动汽车隔离出来.
- 纳米粒子跟踪分析,电子显微镜和共同免疫沉被使用.
主要成果:
- 这三种细胞类型都将FGF2-18kDa分泌到分泌体和EV中.
- 过氧增加了EV总产量,并增强了FGF2-18kDa从HBE细胞在EV中的释放.
- 内化EV将FGF2-18kDa输送到受体细胞核和细胞质中,在那里它与H1.2结合蛋白等蛋白质结合.
- 在Matrigel测定中,FGF2显示出亲血管性活性.
结论:
- 肺细胞,包括上皮细胞,通过EVs输出FGF2-18kDa,特别是在高氧条件下.
- 通过EV介导的FGF2运输为细胞间FGF2信号提供了一条新的途径,可能独立于细胞表面受体.
- 在EVs中的FGF2可能在调节肺损伤,修复和血管生成方面发挥作用.
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