开发和验证一种非侵入性方法来量化人类唾液中的氨基酸
Md Mehedi Hasan1, Mamudul Hasan Razu1, Sonia Akter1
1Bangladesh Reference Institute for Chemical Measurements Dhaka Bangladesh dg.mic@bricm.gov.bd mhrazu@bricm.gov.bd.
RSC advances
|July 16, 2024
概括
唾液是一种优越的,非侵入性的疾病诊断矩阵. 这项研究量化了13种无唾液氨基酸样本,使用LC-MS/MS用于早期癌症检测和治疗研究.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 唾液在采样方面比血液和尿液具有优势,因为它具有非侵入性和易于采集的特性.
- 量化唾液氨基酸可以帮助早期发现疾病,特别是各种恶性瘤.
- 液体染色学-并联质谱学 (LC-MS/MS) 为复杂的生物样本提供了高灵敏度和选择性.
研究的目的:
- 开发和验证一种方法来量化人类唾液中的13种无唾液氨基酸 (SFAA) 样本.
- 通过分析SFAA模式来支持包括癌症在内的疾病的早期临床诊断.
- 确定LC-MS/MS对于唾液中敏感和选择性氨基酸分析的实用性.
主要方法:
- 唾液样本使用液体染色学-并联质谱法 (LC-MS/MS) 分析,其中使用了内置氨基酸列.
- 在正离子模式下使用二进制梯度化和电喷离子电离,用于染色学分离和检测.
- 氨基酸是使用乙尼提取的,量化是使用多重反应监测 (MRM) 模式进行的.
主要成果:
- 该方法证明了对大多数氨基酸的微分子范围内的检测极限 (LOD) 和量化极限 (LOQ) 值的高灵敏度.
- 矩阵匹配的校准曲线显示出极好的线性 (r2 ≥ 0.998),恢复实验证实了准确性 (85-110%).
- 日内和日间精度一般较低 (0.02-7.28%),表明SFAA量化的可靠方法.
结论:
- 开发的LC-MS/MS方法适用于精确和灵敏地量化唾液中的SFFA概况.
- 唾液氨基酸模式显示出作为癌症早期检测和监测的生物标志物的潜力.
- 这种方法可以促进研究新的癌症治疗方法和识别各种疾病的生物标志物.
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