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再组合酶聚合酶放大与侧流生物传感器相结合,用于在肉和牛奶中快速视觉检测Clostridium perfringens

Rui Tian^1,2, Feng Xie^1,2, Yuqing Liu³

¹The Sanya Institute of Nanjing Agricultural University, Yabulun Industrial Park, Yazhou Bay Science and Technology City, Sanya, China.

Frontiers in veterinary science

|July 16, 2024

概括

一种新的重组酶聚合酶放大 (RPA) 和侧流生物传感器 (LFB) 方法快速检测出Clostridium perfringens的食品污染. 这种视觉测试提供了一个快速而准确的现场工具,用于识别这种常见的食源性病原体.

科学领域:

食品微生物学食品微生物学
分子诊断学分子诊断学
生物传感器技术技术

背景情况:

杆菌 (Clostridium perfringens) 是一种主要的无氧病原体,引起食物中毒和动物肠炎.
抗生素耐药性的增加和对抗生素生长促进剂的限制,需要快速的,现场检测方法C. perfringens.
对于C. perfringens检测的传统培养方法是耗时且劳动密集的.

研究的目的:

开发和验证一种快速的视觉检测工具,用于食品矩阵中的Clostridium perfringens.
将复合酶聚合酶放大 (RPA) 与侧流生物传感器 (LFB) 结合起来,用于现场检测C. perfringens.
评估开发的RPA-LFB试验的特异性,敏感性和效率.

主要方法:

设计的原料向C. perfringens的plc基因进行RPA放大.
开发了一种侧向流动生物传感器 (LFB),其中包含一个用于放大产品的探头.
优化了RPA-LFB反应条件,并评估了针对常见的食源性病原体的特异性.
使用人工注射的和牛奶样本评估灵敏度,并与PCR进行比较.

主要成果:

RPA-LFB测定可以在20分钟内完成,并进行视觉结果解释.
该方法表明可接受的特异性和核酸检测极限为100 pg/μL.
在和牛奶中成功检测出C.perfringens,其度分别低至1x10^4 CFU/mL和1x10^3 CFU/mL.

结论:

开发的RPA-LFB试验是一种特定,敏感,快速和简单的C. perfringens检测方法.
LFB测试条的视觉性质使其适合于现场现场测试.
这种方法为监测食品中C. perfringens污染提供了有价值的工具.

关键词:

克洛斯特里 (Clostridium perfringensens) 是一种有毒的植物.食物传播的病原体侧流生物传感器侧流生物传感器在现场检测方法的现场检测方法.复合酶聚合酶放大复合酶.

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