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生物信息学分析了肥胖和胃癌之间的关联

Xiaole Ma¹, Miao Cui², Yuntong Guo¹

¹Department of Gastrointestinal Surgery, First Hospital of Shanxi Medical University, Taiyuan, China.

Frontiers in genetics

|July 16, 2024

概括

肥胖和胃癌有共同的分子通路,主要涉及炎症和免疫反应. 这项研究确定IL6和CCL4是关键基因,为共发病机制提供了洞察力.

科学领域:

基因组学和生物信息学
分子生物学分子生物学
在瘤学瘤学.

背景情况:

肥胖和胃癌 (GC) 是全球重要的健康问题,患病率越来越高.
连接肥胖和GC的潜在分子机制在很大程度上仍然不清楚,尽管有证据表明存在强烈的关联.

研究的目的:

确定肥胖和胃癌中共享的分子机制和关键调节基因.
构建一个涉及枢纽基因,转录因子 (TFs) 和两种条件共同的微RNA (miRNAs) 的调节网络.

主要方法:

使用了基因表达综合 (GEO) 数据集 (GSE94752,GSE54129) 中的基因表达特征.
在共享的差异表达基因上进行基因本体学和KEGG通路分析.
在Cytoscape中使用MCODE和CytoHubba构建了蛋白质-蛋白质相互作用 (PPI) 网络和识别的枢纽基因.
分析了TF-miRNA-mRNA调节网络,并使用独立数据集验证了枢纽基因.

主要成果:

确定了246个在肥胖和GC之间分别表达的共享基因,重点关注炎症和免疫通路.
发现了9个潜在的枢纽基因,通过验证证实IL6和CCL4具有重要意义.
建立了一个监管网络,强调RELA和NFKB1作为关键的TF,并将has-miR-195-5p和has-miR-106a-5p作为关键的miRNA.

结论:

生物信息分析显示IL6和CCL4是潜在地将肥胖和胃癌联系起来的关键枢纽基因.
已识别的TF-miRNA-mRNA网络为共享的分子机制提供了更深入的理解.
这些发现为进一步研究肥胖和GC共同疾病的潜在目标提供了潜在的目标.

关键词:

生物信息学是一种生物信息学.不同表达的基因.胃癌胃癌是一种胃癌.核心基因核心基因核心基因肥胖肥胖肥胖肥胖肥胖肥胖肥胖肥胖

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